Detection Of Human Cytomegalovirus In Qualified Blood Donors

Objective:Human cytomegalovirus(HCMV) is the largest DNA virus of the genome in Beta subfamilies of Herpesviridae, which has a closed relationship with the transfusion of human. It has a biological characteristic of latent- active infection what we usually called latent infection. Similar to other Herpesviridae, HCMV is capable of producing an active infection as well as maintaining a lifelong latent infection. The virus infects humans at any age but is traditionally considered to be an innocuous virus except in cases of immunosuppression(Like: transplant patients, Fetus intrauterine transfusion, low birth weight infants and CMV serological test negative pregnant women). during the process of transfusion the immunosuppred people is more easily be infected and resulted in HCMV infection and HCMV disease. HCMV infection is endemic, which could be detected in 70–90 % of the adult population, in 40 % occurring during the first year of life [1]. HCMV infects a wide range of tissues, including the brain. Following primary infection, which is usually asymptomatic, HCMV resides in a latent state in many cell types, include the neural stem cells. During the process of transfusion, HCMV is more easily be infected and resulted in HCMV infection and HCMV disease, Infusion of blood with HCMV seronegative and deleukocytoed could reduce the occurrence of it, though it is not efficient to everybody. The HCMV infection is an important reason to the morbidity and the mortality during patients with transplantation of allogeneic hematopoietic stem cell(allo-HSCT). For understanding the donors situation of Carrying HCMV, so as to provide favorable protection for the safety of blood transfusion. In this experiment, two methods,PCR and ELISA were used to detect the infection of HCMV of the 70 donors, and the sensitivity of this two kind methods were compared.Methods:70 cases of donors were randomly selected, including male 37 cases, female 33 cases, by using PCR gel electrophoresis and ELISA method to detect the positive rate of HCMV DNA with the antibody of Ig G and the consistent rate of these two ways, we used EDTA K2 to avoid the specimens agglutinate. So as to observe the positive rate and the consistent rate of these two ways.Results:During the PCR gel electrophoresis test, there were 15 cases of HCMV DNA positive, the positive rate was 21.43%; while in the ELISA test, 11 cases of CMV antibody Ig G was positive, the positive rate was 15.71%. we regard the PCR as the gold standard,The sensitivity of the PCR method is100%, while the ELISA method is 53.33%;there are8 cases of HCMV-Ig G positive samples in15 cases of HCMV DNA positive samples, the coincidence rate was 53.33%, 52 cases of HCMV-Ig G negative specimens in 55 cases of HCMV DNA negative specimens, the coincidence rate was 94.55%.Conclusions:The positive rate of the HCMV DNA which used the nucleic acid detection is higher the antibody Ig G of HCMV, and the sensitivity of the nucleic acid detection was more higher than that of ELISA detection by compared. the risk of infection HCMV is existed,It is necessary to check the HCMV of donors blood carrying which will be used during the blood transfusion for the high risk group, while using the leukoreducted and seronegative blood products which could reduce the infection of HCMV for the high risk group is an idea method for the moment.