The Protective Effect Of Hypericum Perforatum.L On Acute Immunological Liver Injury And Intestinal Epithelial Barrier Injury Accompanied In Mice

There are a large number of people with liver disease in China. The number of hepatitis B surface antigen carriers is about 1.2 billion. It can further develop into liver fibrosis, cirrhosis and even hepatocellular carcinoma without timely regular treatment. Currently we advocate comprehensive therapy in clinical. And traditional Chinese medicine has made some progress in this area. Hypericum Perforatum.L(HPL), also known as St John’s wort, as guttiferae hypericum perennial herbaceous plants, has a variety of activation. There are various links between liver and intestine. Studies have found that liver injury is often accompanied by intestinal epithelial barrier dysfunction.Objective To investigate the protective effect of HPL on concanavalin A(Con A)-induced acute immunological liver injury in mice and the change of intestinal epithelial barrier in the process.Methods Sixty ICR mice were randomly divided into six groups: normal group, model group, HPL(25, 50, 100 mg/kg) groups and positive control group(dexamethasone, 2.5 mg/kg). The model of mice acute immunological liver injury was induced by tail intravenous injecting with Con A(20 mg/kg) on day 10 after treatment with different concentrations of HPL. After 8 to 12 h, mice were weighed and sacrificed. The liver and spleen index were calculated, the level of ALT, AST, γ-GT, TBIL, GLB in serum, SOD, MDA, TLR4 in liver tissues, the level of DAO in intestinal tissues, and ZO-1, occludin, TLR4, GFAP, S100 in intestinal tissues were tested. Additionally, the hepatic pathological changes were observed.Results Compared with model group, the liver and spleen index, the level of ALT, AST, γ-GT, TBIL, GLB in serum and the content of MDA and TLR4 in liver tissue were decreased, the SOD activity was increased in HPL groups. The activity of DAO and the content of ZO-1, occludin, TLR4, GFAP, S100 in intestinal tissues was increased in HPL groups. Moreover, the hepatic and intestinal pathological injury was obviously alleviated after treatment with HPL.Conclusion HPL had obviously protective effects on acute immunological liver injury and intestinal epithelial barrier injury inmice.