Effects Of TGF-beta1 Insulin Composite Gel On Deep Second Degree Scald Wound Healing In Diabetic Rats

Objective Observe the effects of TGF-betal insulin composite gel on deep second degree scald wound healing in diabetic rats.Methods Carbomer 980 gel as the martix, add TGF-betal 2 ug and insulin 1600 U into the Carbomer 980 gel so that to get the blank gel、insulin ge1、TGF-β1 gel and TGF-β1-insulin composite gel. Totally 75 male clean SD rats were fed with well, weighing between 200g to 290g,The diabetic rats model was designed by abdominal injection of STZ (55 mg/kg),and then all the rates were burned became the deep second degree scald wound. The 75 rates were randomly divided into five groups(A、B、C、 D、E), free control (Con) group (n=15) Diabetes group (n=15, Insulin group(n=15),TGF-β1 gel group (n=15), TGF-β1-insulin composite gel group (n=15).Different kinds of gel were daubed on the wound of the rates to observe the effect of the skin healing rate, on the 3th、7th、llth、15th、and 21th day, healing rate is calculated at each time point, rats in each group were sacrificed three, at each point day, and then full-thickness skin samples were taken for histological and immunohistochemistry staining.Result All the carbomer gel we got were Colorless and transparent.During the period of the experiment, all the rates were survived, there is a small difference in the five groups in the first three days, at the 7th、11th、15th and 21th day,we can see that, the healing rare in the TGF-β1 gel group was the highest (76.69%), however, the Diabetes group was the minimum, which compared with the other four groups (P<0.05),In the five point time, we find that, the TGF-β1-insulin composite gel group with the largest number of capillaries, and with the highest densities of I and III collagen, but the Diabetes group was the minimum, In the five groups we can observe that, the number of capillaries、the densities of I and III collagen, all of them started to increase from the third day, and arrived to the top at the 15th day, the Diabetes group compared with the other four groups has the obvious advantages (P<0.05). TGF-β1 gel group compared with TGF-β1-insulin composite gel group was significantly (P<0.05).Conclusion 1.Diabetes rates model is helpful to discuss the mechanism of the Diabetes, and TGF-β1-insulin composite gel is good for the chronic and refractory wound.2. TGF-β1-insulin composite gel can promote cell fibrosis and promote functions of cells, on one hand which can promote wound repair tissue, improve wound healing rate. On the other hand which will increase the number of capillaries、the densities of I and III collagen.