The Role Of SDF-1 In The Proliferation And Invasion Of Ovarian Cancer Cells Through Activation Of PI3K/Akt Signaling Pathway

ObjectiveBy Observing the effect of stromal cell-derived factor 1(SDF-1) and phosphatidylinositol 3-kinase / protein kinase B(PI3K/Akt) signaling pathway inhibitor LY294002 on the proliferation and invasion of ovarian cancer cells SKOV3 and CAOV3 to explore that whether PI3 K / Akt signaling pathway has a role in the process of the effect of SDF-1 proliferation and invasion of epithelial ovarian cancer cell. Methods1.Ovarian cancer cells SKOV3 and CAOV3 were divided into four groups respectively.(1) control group,(2) group 1(100ng/ml SDF-1),(3) group 2(50μmol/L LY294002),(4) group 3(deal ovarian cancer cells for half an hour with 50μmol/L LY294002 before using 100ng/ml SDF-1).2.Methyl thiazol tetrazolium(MTT) assay was used to detect the proliferation ability of ovarian cancer cells SKOV3 and CAOV3, when they were treated by different drugs at 24 h, 48 h and 72 h.3.Using Transwell invasion method to test the invasion ability of ovarian cancer cells SKOV3 and CAOV3,when they were treated by different drugs at 48 h.4.Western Blot was used to detect the degree of phosphorylation of Akt and the secretion of matrix metalloproteinase-9 in ovarian cancer cells SKOV3 and CAOV3, when they were treated by different drugs at 48 h. Results1. The difference of the results of MTT in ovarian cancer cell SKOV3 had no statistically significant, when they were treated with different drugs for 24 hours(P>0.05). When we compared the SDF-1 group(group 1) with the control group, which were treated with different drugs for 48 and 72 hours, we could see that SDF-1 could significantly promote the proliferation of ovarian cancer cell SKOV3(P<0.05). While, when we compared the LY294002 group(group 2) and the group of dealing ovarian cancer cell SKOV3 with 50μmol/L LY294002 for half an hour before using 100ng/ml SDF-1(group 3) with the control group respectively, we could see that the proliferation of ovarian cancer cell SKOV3 was significantly reduced(P<0.05). Moreover, we could see that the role of SDF-1 in the promoting of proliferation in ovarian cancer cell SKOV3 was significantly inhibited in the comparing of group 3 and group 1(P<0.05). And the difference was not statistically significant when we compared group 3 and group 2(P>0.05).The variation of MTT results in ovarian cancer cell CAOV3 were similar when they were measured respectively at 24 h, 48 h, and 72 h. we could see that SDF-1 could significantly promote the proliferation of ovarian cancer cell CAOV3, when we compared group 1 and control group(P<0.05). While, when we compared group 2 and group 3 with the control group respectively, we could see that the proliferation of ovarian cancer cell CAOV3 was significantly reduced(P<0.05). Moreover, we could see that the role of SDF-1 in the promoting of proliferation in ovarian cancer cell CAOV3 was significantly inhibited in the comparing of group 3 and group 1(P<0.05). And the difference was not statistically significant when we compared group 3 and group 2(P>0.05).2. We could see that SDF-1 could significantly promote the invasion of ovarian cancer cells SKOV3 and CAOV3, when we compared group 1 with control group(P<0.05). While, when we compared group 2 and group 3 with the control group respectively, we could see that the invasion of ovarian cancer cells SKOV3 and CAOV3 was significantly reduced(P<0.05). Moreover, we could see that the role of SDF-1 in the promoting of invasion in ovarian cancer cells SKOV3 and CAOV3 was significantly inhibited in the comparing of group 3 and group 1(P<0.05). And the difference was not statistically significant when we compared group 3 and group 2(P>0.05).3.The expression changs of Akt, phosphorylation of Akt and matrix metalloproteinase-9 in ovarian cancer cells SKOV3 and CAOV3 were similar, when they were treated with different drugs for 48 h. It had a certain degree of the activation of PI3 K / Akt signaling pathway and the secretion of MMP-9 in ovarian cancer cells SKOV3 and CAOV3 at baseline. The expression of Akt in different groups was no significant difference in the two ovarian cancer cells(P>0.05).We could see that SDF-1 could significantly promote the expression of p-Akt and MMP-9 in ovarian cancer cells SKOV3 and CAOV3, when we compared group 1 with control group(P<0.05). While, when we compared group 2 and group 3 with the control group respectively, we could see that the expression of p-Akt and MMP-9 in ovarian cancer cells SKOV3 and CAOV3 was significantly reduced(P<0.05). Moreover, we could see that the role of SDF-1 in the promoting of the expression of p-Akt and MMP-9 in ovarian cancer cells SKOV3 and CAOV3 was significantly inhibited in the comparing of group 3 and group 1(P<0.05). And the difference was not statistically significant when we compared group 3 and group 2(P>0.05). Conclusion1. SDF-1 could promote the proliferation and invasion in human ovarian cancer cells SKOV3 and CAOV3.2. As the inhibitor of PI3K/Akt signaling pathway, LY294002 could inhibite the proliferation and invasion in human ovarian cancer cells SKOV3 and CAOV3.3. After blocking the PI3K/Akt signaling pathway, the promoting of SDF-1 to the proliferation and invasion in human ovarian cancer cells SKOV3 and CAOV3 was inhibited.4. PI3K/Akt signaling pathway involved in the stimulation of the secretion of MMP-9 in human ovarian cancer cells SKOV3 and CAOV3.5. The role of SDF-1 in the promoting of proliferation and invasion in human ovarian cancer cells SKOV3 and CAOV3 maybe achieved by the the increased expression of MMP-9, which may increase the expression by the activation of PI3K/Akt signaling pathway.