Study On The Clinical Significance Of Hsp90 And VEGF In Serum Of Patients With Non-small Cell Lung Cancer And Its Correlation With TCM Syndrome Type

Background and Objective: Heat shock protein(HSPs) is a kind of protein substances produced by the body after stress reaction. HSP90 alpha as a member of the heat shock protein family, has been shown to increase in non small cell lung cancer, gastric cancer, pancreatic cancer, glioma and meningioma and so on many kinds of tumor tissues and cells content. VEGF can strongly promote vascular endothelial cell mitosis of tumor angiogenesis, is the most effective stimulating factor. Both with lung cancer occurrence, development are closely linked. At present, Chinese medicine treatment of lung cancer, one is based on dialectical treatment mainly, one is the combination of dialectical and disease. But the Chinese dialectical theory of governance in the application of tumor disease is difficult to fully reflect the common treatment of majority cases, and even the existence of unlicensed discernible; card discernible, resolution and error phenomenon. The development of modern evidence-based medicine to Chinese dialectical theory of governance provides a very good development model. In collecting the information of four diagnostic methods at the same time, combined with modern laboratory tests such as tumor marker, immunological indexes of Chinese dialectical theory of governance, it is the beneficial attempt and exploration.Methods: The extraction of 80 cases of hospitalized patients with NSCLC in healthy people over the same period, physical examination center of outpatient in 30 cases and the control. All of the subjects in the fasting state, extraction 3ml blood stored in EDTA tubes, mixing evenly, taking the serum 1ml 3000 R centrifugal 20 min at 4 DEG C in a centrifuge. Number after freezing at-80 deg.c refrigerator. To detect the serum levels of VEGF and Hsp90 alpha content by ELASE method. At the same time the dialectical typing of 80 cases of NSCLC patients with traditional Chinese medicine according to the unified standard.Results: 1. 30 cases of healthy people serum Hsp90(pg/ml) 61.799 + 30.837, and the samples of serum Hsp90 levels in lung cancer group is 385.425 + 173.484, significantly higher than that of the normal control group(P < 0.05).VEGF serum levels of 2 lung cancer(pg/ml) 392.519 + 159.228, the serum level of VEGF of control group is 82.438 + 38.329, the difference was statistically significant(P < 0.05).3. I ~ II phase III, IV stage, serum Hsp90 concentration(pg/ml) of 301.001 + 151.757, 449.835 + 163.602, 466.932 + 157.471, the difference was statistically significant(Kruskal- Wallis rank sum test, P <0.05). Further analysis using Spearman rank sum test levels of HSP90 alpha and TNM in serum of staging relevance, showed they were positively correlated(correlation coefficient r=0.425, P <0.001). Prompt staging more late, the higher the concentration of serum Hsp90.4. the concentration of serum VEGF(pg/ml) in stage I to II, III, IV period distribution of each period was 308.529 + 130.812, 421.801 + 194.887, 483.113 + 156.330, the difference was statistically significant(P <0.05). Analysis of TNM stage were significantly correlated with serum VEGF levels(P <0.05).5. Qi deficiency and phlegm dampness syndrome, qi stagnation and blood stasis, yin deficiency and toxic heat syndrome, Qi and yin deficiency group, the serum Hsp90 levels(pg/ml) were 301.368 + 153.180, 408.932 + 150.550, 479.515 + 194.372, 342.594 + 155.558, the difference was statistically significant(ANOVA, F=4.221, P <0.01), and serum Hsp90 concentrations from high to low in order to Yin toxin heat syndrome stasis >Syndrome of qi stagnation and blood stasis > Syndrome of deficiency of Qi and Yin two>Qi deficiency and phlegm dampness syndrome. LSD-t comparison between groups can be seen: Qi deficiency and phlegm dampness syndrome group and Yin toxin heat syndrome of qi stagnation and blood stasis group, there were significant differences between group comparison(P <0.05); no significant differences between the groups of deficiency of both qi and Yin and stagnation of Qi and blood stasis group(P>0.05), there is significant difference in comparison with Yin deficiency and heat toxin group(P< 0.05); Yin deficiency and toxic heat group and qi stagnation and blood stasis group, Qi deficiency and phlegm dampness and deficiency of both qi and Yin group respectively, there were no significant difference(P >0.1).6. the concentration of serum VEGF in four groups of syndrome type distribution of 317.456 + 138.936, 490.429 + 174.351, 432.662 + 147.292, 357.830 + 144.339, the difference was statistically significant(ANOVA, F=5.739, P <0.01). The concentration of serum VEGF from high to low are qi stagnation and blood stasis type > Yin toxin heat syndrome > Qi Yin deficiency two> Qi deficiency and phlegm dampness syndrome. LSD-t group analysis results: Qi deficiency and phlegm dampness syndrome group and qi stagnation and blood stasis group, yin deficiency heat toxin syndrome group comparisons showed statistical difference(P <0.05), showed no significant difference compared with deficiency of both qi and Yin group(P>0.05); there were no significant differences in Yin deficiency and heat toxin group and qi stagnation and blood stasis group, deficiency of both qi and Yin Syndrome group(P >0.05), there was significant difference between the syndrome of qi stagnation and blood stasis group and the Qi and yin deficiency syndrome group.7. Correlation between TCM syndrome type and TNM staging,By the Pearson correlation analysis, the correlation coefficient r=0.418,χ2 =7.341, P=0.291. Show that the non small cell lung cancer patients with various TCM type and TNM staging was not related. See Table 4 for more information.Conclusion: 1. lung cancer patients serum VEGF and Hsp90 levels were significantly higher than those in normal people.2. lung cancer patients with elevated serum VEGF and Hsp90 levels with disease progression.3. The first diagnosed lung cancer patients with serum Hsp90 concentration from high to low according to Yin toxin heat syndrome of stagnation of Qi and blood stasis > > two > Qi and yin deficiency syndrome of qi deficiency and phlegm dampness syndrome. The concentration of serum VEGF from high to low are qi stagnation and blood stasis type > Yin toxin heat syndrome of Qi and yin deficiency syndrome of > two > Qi deficiency and phlegm dampness syndrome.4. There is no relation ship between TCM syndrome types and TNM stages in the first diagnosis of non-small cell lung cancer patients.