| Objective: detecte serum zinc(Zn), copper(Cu), selenium(Se), magnesium(Mg) elements of leukemia patient to investigate the relationship of zinc, copper, selenium, magnesium levels and diagnosis, treatment and prognosis of Leukemia patient.Method:1 randomly selected 122 leukemia patients with histologically diagnosed in Hematology of our hospital as Experimental group in this study, while selecting 100 normal control people who to Examination in medical centers of our hospital at the same period, the clinical data and blood samples were collected. 122 cases in Experimental group and 100 cases in normal control group were detected zinc, copper, selenium, magnesium content of serum by fluorescence spectrophotometer.2 statistical methods:Description and analysis of the data using SPSS13.0. General information by statistical description, measurement data by(x_±s), first to make normality and homogeneity of variance test on all groups, if the data subject to normality and homogeneity of variance, using the t test; measurement data used to compare two samples use t test. If the information did not subject to the homogeneity of variance and normality, then use Kruskal-Wallis H, pairwise comparisons using rank analysis of variance conversion. Significance level α = 0.05, P <0.05, the difference was statistically significant.Results:1 Compare blood levels of trace elements in 122 cases of leukemia patient group and the control group, the results showed that leukemia patient group zinc, selenium, magnesium content lower than the control group, respectively, zinc(12.68 ± 4.78μmol / L ratio of 15.01 ± 4.02μmol / L, P <0.05), selenium(0.86 ± 0.07μmol / L ratio of 1.70 ± 0.26μmol / L, P <0.05), magnesium(0.96 ± 0.10μmol / L ratio of 1.34 ± 0.09μ mol / L, P <0.05), and leukemia was higher copper content(26.02 ± 8.78μmol / L ratio of 15.18 ± 3.98μmol / L,P<0.05), the differences were statistically significant.2 We compared serum zinc, selenium, magnesium and copper levels in patients with Different gender of leukemia. The results showed that the content of various trace elements in no significant difference between men and women(P> 0.05)3 The leukemia respectively compared with the control group, we found that acute myeloid leukemia and Chronic lymphocytic leukemia was statistically significant difference compared with the control group(P <0.05). The research collected 26 cases of acute myelogenous leukemia, 35 cases of acute lymphoblastic leukemia, 31 cases of chronic lymphoblastic leukemia, 30 cases of chronic myeloid leukemia. Then respectively compare with the control group, the results showed that compared with the control group, acute myelogenous leukemia average serum zinc lower than the control group(10.89 ± 3.76 μmol / L ratio of 15.01 ± 4.02μmol / L, P <0.001), selenium lower than the control group(0.75 ± 0.12μmol / L ratio of 1.70 ± 0.26μmol / L, P <0.05), serum trace element magnesium lower than the control group(0.72 ± 0.10μmol / L ratio of 1.34 ± 0.09μmol /L,P<0.05), and copper was higher(25.23 ± 8.02μmol / L ratio of 15.18 ± 3.98μmol / LP <0.05), and the differences were statistically significant. Acute lymphoblastic leukemia group compared with normal control group, the results showed that acute lymphoblastic leukemia mean serum zinc lower than the control group(13.78 ± 4.85μ mol / L ratio of 15.01 ± 4.02, P = 0.28), Selenium lower than the control group(1.01 ± 0.06μmol / L ratio of 1.70 ± 0.26, P = 0.041), serum trace element magnesium lower than the control group(0.84 ± 0.07μmol / L ratio of 1.34 ± 0.16μmol / L, P = 0.038), while Copper was higher(24.87 ± 7.45μmol / L ratio of 15.18 ± 3.98μmol / L, P = 0.0021), but only selenium and copper statistically significant difference between the two groups. Compared with normal control group, chronic lymphocytic leukemia mean serum zinc lower than the control group(11.04 ± 4.01μmol / L ratio of 15.01 ± 4.02μmol / L, P = 0.012), selenium than the control group(0.69 ± 0.09μmol / L ratio of 1.70 ± 0.26μmol / L, P = 0.0049), serum trace element magnesium lower than the control group(0.76 ± 0.11μmol / L ratio of 1.34 ± 0.09μmol / L, P = 0.017), while Copper high in the control group(28.56 ± 5.97μmol / L ratio of 15.18 ± 3.98μmol /L, P <0.001), the differences were statistically significant. Chronic myelogenous leukemia group compared with the control group, zinc lower than the control group(13.67 ± 5.78μmol / L ratio of 15.01 ± 4.02μmol / L, P = 0.34), selenium lower than the control group(0.89 ± 0.14μ mol / L ratio of 1.70 ± 0.26μmol / L, P = 0.035), serum trace element magnesium lower than the control group(0.89 ± 0.09μmol / L ratio of 1.34 ± 0.16μmol / L, P = 0.040), and trace elemental copper was higher(27.53 ± 6.32μmol / L ratio of 15.18 ± 3.98μmol / LP <0.001), but only selenium and copper statistically significant difference between the two groups.4 We tested levels of trace elements before and after remission induced by chemotherapy of Patients with leukemia, the results show leukemia patients, compared before chemotherapy remission with after chemotherapy a month, the serum zinc(13.05 ± 3.21μmol / L ratio of 12.68 ± 4.78μmol / L, P = 0.12), selenium(0.86 ± 0.07μmol / L ratio of 1.01 ± 0.13μmol / L, P = 0.034), magnesium(0.96 ± 0.10μmol / L ratio of 1.13 ± 0.21μmol / L, P = 0.042) were increased compared with before chemotherapy remission, but the copper(26.02 ± 8.78μmol / L ratio of 23.26 ± 5.89μmol / L, P = 0.108), was declined but all the differences were not statistically significant. Three months after the remission induced by chemotherapy selenium content significantly increased than before treatment(1.52 ± 0.16μmol / L ratio of 0.86 ± 0.07μmol / L, P = 0.017), while the copper content significantly decreased than before treatment(17.04 ± 3.45μmol / L ratio of 26.02 ± 8.78μmol / L, P = 0.022), and the differences were statistically significant(P <0.05), and zinc(13.86 ± 2.98μmol / L ratio of 12.68 ± 4.78μmol / L, P = 0.087) and magnesium(1.20 ± 0.30μmol / L ratio of 0.96 ± 0.10μmol / L, P = 0.034) were increased, but the differences were not statistically significant. Further tracking, six months after the remission induced by chemotherapy selenium continues to rise, with the normal control group no significant difference(1.72 ± 0.31μmol / L ratio of 1.70 ± 0.26μmol / L, P = 0.35), and serum copper continued to decline, with the normal control group had no significant difference(14.8 ± 4.03μmol / L ratio of 15.1 ± 3.98μ mol / L, P = 0.42).Conclusion: Serum zinc, copper occurs, selenium, magnesium is closely related to the development of leukemia,through reasonable regulation of serum zinc, copper, selenium, magnesium levels, may have important prevention of leukemia; strengthen the research on trace elements, contribute to the diagnosis and treatment of leukemia. |
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