| Objective: To explore whether glutamine could reduce or delay the oxidative stress condition of sepsis mice and then play a protective role in the oxidative stress condition of critical disease.Methods:1 Animal groups and building methods: 96 kunming mice were divided into four groups randomly, every group which had 24 kunming mice respectively was named blank group, model group, glutamine low dose group, glutamine high dose group.The blank group was served as control by intraperitoneal injection saline,and the model group and glutamine group whose Injection metering is 5 mg/kg produced an experimental endotoxin model by intraperitoneal injection endotoxin;After the success of the model,the model group was injected saline by tail vein,the low dose glutamine group was injected glutamine whose dose is 0.75g/kg,the high dose glutamine group was injected glutamine whose dose is 1.5g/kg,at the 1 h, 6 h and 12 h after injection, blood samples were drawn from orbit and then killed mice.The mice liver was dissected and separated,and then was frozen with liquid nitrogen rapidly.At last all the samples were placed into refrigerator at-80 ℃.2 The detection method of glutathione peroxidase, superoxide dismutase and malondialdehyde dismutaseThe oxidative stress index including superoxide dismutase, glutathio n peroxidase and malonaldehyde of Serum and liver tissue homogenate from the four groups were measured.Berore testing sample, firstly, these sample were taken out from-80 ℃ refrigerator and then placed into 4℃refrigerator for thawing.Secondly,Animal tissue block was rinsed for removing blood with cold saline solution,wiped dry,accurate weighedup to 0.1g and then plusing 0.9 ml saline plced in the slurry pipe to the weight(g):volume(ml)=1:9 proportion.The tissue block was fleetly cut into pieces with an eye small cut in chilled water.In chilled water homogenate of tissue block was grinded manually at a speed of 30s/tim ewith an interval 30 seconds.According to the above methods,repeat grin ding 6~8 times and then make into 10% tissue homogenate.The 10% tissue homogenate well done was centrifuged 15 min at a speed of 2500~3000r/min with supercentrifuge.Moderate 10% homogenate taken was diluted into 1% homogenate according to volume ratio 1:9 for testing. In order to guarantee the experimental accuracy, homogenate was deter mined at the same day.3 The data was type-in with Excle 2007 and Description and analy sis of statistical data was finished with SPSS13.0.The statistic description of measurement data was given in the form of x_±s,The data of four g roups was tested by normality and homogeneity of variance,If the data of each group was satisfied with both normality and homogeneity of variance,parameter testing method would be chosen.The comparison o f two independent samples,the method of paired samples t test would b e chosen,The comparison of three independent samples,the method of one-way analysis of variance would be chosen.If the data could not meet both the methods of normality and homogeneity of variance, Kruskal-Wallis H nonparametric test would be chosen,the further comparison between any two means, Nemenyi test would be chosen. Inspection level,α=0.05, P<0.05, the difference is statistically significant.Results:1 The comparison of SOD index level1.1 At 1h, the comparison of SOD index level1.1.1 The comparison of serum SOD index level:the comparison of SOD levels of four groups,there was statistically significant between the differences,(P<0.05).The comparison between any two means,there w as no statistically significant between the differences in model group than that in glutamine low dose group and glutamine high dose group at SODlevel(P>0.05);there was no statistically significant between the di fferences in glutamine low dose group than that in glutamine high dose group at SOD level(P>0.05).1.1.2 The comparison of liver SOD index level:the comparison of SOD levels of four groups,there was statistically significant between the diff erences,(P<0.05).The comparison between any two means,there was no statistically significant between the differences in model group than that in glutamine low dose group and in glutamine high dose group at SOD level(P>0.05);there was no statistically significant between the differences in glutamine low dose group than that in glutamine high dose group at SOD level(P>0.05).1.2 At 6h, the comparison of SOD index level1.2.1 The comparison of serum SOD index level:the comparison of SOD levels of four groups,there was statistically significant between the differences,(P<0.05).The comparison between any two means,there w as statistically significant between the differences in model group than t hat in blank group,in glutamine low dose group and in glutamine high dose group at SOD level(P<0.05);There was statistically significant between the differences in blank group than that in glutamine low dos e group and in glutamine high dose group at SOD level(P<0.05);There was no statistically significant between the differences in glutamine low dose group than in glutamine high dose group(P>0.05).1.2.2 The comparison of liver SOD index level:the comparison of SOD levels of four groups,there was statistically significant between the diff erences,(P<0.05).The comparison between any two means,there was statis tically significant between the differences in model group than that in b lank group(P<0.05);There was no statistically significant between the di fferences in model group than that in glutamine low dose group and in glutamine high dose group at SOD level(P>0.05);There was no statistica lly significant between the differences in glutamine low dose group and in glutamine high dose group at SOD level(P>0.05);1.3 At 12 h, the comparison of SOD index level1.3.1 The comparison of serum SOD index level :the comparison of SO D levels of four groups,there was statistically significant between the d ifferences,(P<0.05).The comparison between any two means,there was sta tistically significant between the differences in model group than that i n blank group,in glutamine low dose group and in glutamine high dose group at SOD level(P<0.05);There was no statistically significant betwe en the differences in glutamine low dose group and in glutamine high dose group at SOD level(P>0.05);1.3.2 The comparison of liver SOD index level:the comparison of SOD levels of four groups,there was statistically significant between the diff erences,(P<0.05).The comparison between any two means,there was statis tically significant between the differences in model group than that in b lank group,in glutamine low dose group and in glutamine high dose gro up at SOD level(P<0.05);There was no statistically significant between t he differences in glutamine low dose group and in glutamine high dose group at SOD level(P>0.05);2 The comparison of GSH-Px index level2.1 At 1h, the comparison of GSH-Px index level2.1.1 The comparison of serum GSH-Px index level:the comparison of GSH-Px levels of four groups,there was statistically significant between the differences,(P<0.05).The comparison between any two means,there w as statistically significant between the differences in model group than t hat in blank group at GSH-Px level(P<0.05);There was no statistically s ignificant between the differences in model group than that in glutamine low dose group and in glutamine high dose group at GSH-Px level(P>0.05);There was no statistically significant between the differences in glut amine low dose group and in glutamine high dose group at GSH-Px le vel(P>0.05);2.1.2 The comparison of liver GSH-Px index level:the comparison of G SH-Px levels of four groups,there was statistically significant between the differences,(P<0.05).The comparison between any two means,there wa s statistically significant between the differences in model group than th at in blank group at GSH-Px level(P<0.05);There was no statistically si gnificant between the differences in model group than that in glutaminel ow dose group and in glutamine high dose group at GSH-Px level(P>0.05);There was no statistically significant between the differences in glut amine low dose group and in glutamine high dose group at GSH-Px le vel(P>0.05);2.2 At 6h, the comparison of GSH-Px index level2.2.1 The comparison of serum GSH-Px index level:the comparison of GSH-Px levels of four groups,there was statistically significant between the differences,(P<0.05).The comparison between any two means,there w as statistically significant between the differences in model group than t hat in blank group,in glutamine low dose group and in glutamine high dose group at GSH-Px level(P<0.05);there was statistically significant be tween the differences in blank group than that in glutamine low dose g roup and in glutamine high dose group at GSH-Px level(P<0.05);There was no statistically significant between the differences in glutamine low dose group and in glutamine high dose group at GSH-Px level(P>0.05);2.2.2 The comparison of liver GSH-Px index level:the comparison of G SH-Px levels of four groups,there was statistically significant between t he differences,(P<0.05).The comparison between any two means,there wa s statistically significant between the differences in model group than th at in blank group at GSH-Px level(P<0.05);There was no statistically si gnificant between the differences in blank group than that in glutamine low dose group and in glutamine high dose group at GSH-Px level(P>0.05);There was no statistically significant between the differences in glut amine low dose group and in glutamine high dose group at GSH-Px le vel(P>0.05);2.3 At 12 h, the comparison of GSH-Px index level2.3.1 The comparison of serum GSH-Px index level:the comparison of GSH-Px levels of four groups,there was statistically significant between the differences,(P<0.05).The comparison between any two means,there w as statistically significant between the differences in model group than t hat in blank group,in glutamine low dose group and in glutamine high dose group at GSH-Px level(P<0.05);There was no statistically significan t between the differences in glutamine low dose group and in glutamine high dose group at GSH-Px level(P>0.05);2.3.2 The comparison of liver GSH-Px index level:the comparison of G SH-Px levels of four groups,there was statistically significant between t he differences,(P<0.05).The comparison between any two means,there wa s statistically significant between the differences in model group than th at in blank group,in glutamine low dose group and in glutamine high d ose group at GSH-Px level(P<0.05);There was statistically significant bet ween the differences in glutamine low dose group and in glutamine hig h dose group at GSH-Px level(P<0.05);3 The comparison of MDA index level3.1 At 1h, the comparison of MDA index level3.1.1 The comparison of serum MDA index level:the comparison of M DA levels of four groups,there was statistically significant between the differences,(P<0.05).The comparison between any two means,there was s tatistically significant between the differences in model group than that i n blank group at MDA level(P<0.05);there was no statistically significan t between the differences in model group than that in glutamine low do se group and in glutamine high dose group at MDA level(P>0.05);Ther e was no statistically significant between the differences in glutamine lo w dose group and in glutamine high dose group at MDA level(P>0.05);3.1.2 The comparison of liver MDA index level:the comparison of MD A levels of four groups,there was statistically significant between the d ifferences,(P<0.05).The comparison between any two means,there was sta tistically significant between the differences in model group than that inblank group at MDA level(P<0.05);there was no statistically significant between the differences in model group than that in glutamine low dose group and in glutamine high dose group at MDA level(P>0.05);There w as no statistically significant between the differences in glutamine low d ose group and in glutamine high dose group at MDA level(P>0.05); 3.2 At 6h, the comparison of MDA index level3.2.1 The comparison of serum MDA index level:the comparison of M DA levels of four groups,there was statistically significant between the differences,(P<0.05).The comparison between any two means,there was n o statistically significant between the differences in model group than th at in blank group,in glutamine low dose group and in glutamine high d ose group at MDA level(P>0.05);There was no statistically significant b etween the differences in glutamine low dose group and in glutamine hi gh dose group at MDA level(P>0.05).3.2.2 The comparison of liver MDA index level:the comparison of MD A levels of four groups,there was statistically significant between the d ifferences,(P<0.05).The comparison between any two means,there was sta tistically significant between the differences in model group than that in blank group at MDA level(P<0.05);there was statistically significant bet ween the differences in model group than that in glutamine low dose g roup and in glutamine high dose group at MDA level(P<0.05);There wa s no statistically significant between the differences in glutamine low do se group and in glutamine high dose group at MDA level(P>0.05);3.3 At 12 h, the comparison of MDA index level3.3.1 The comparison of serum MDA index level:the comparison of M DA levels of four groups,there was statistically significant between the differences,(P<0.05).The comparison between any two means,there was s tatistically significant between the differences in model group than that i n blank group,in glutamine low dose group and in glutamine high dose group at MDA level(P<0.05);There was no statistically significant between the differences in glutamine low dose group and in glutamine high dose group at MDA level(P>0.05).3.3.2 The comparison of liver MDA index level:the comparison of MD A levels of four groups,there was statistically significant between the d ifferences,(P<0.05).The comparison between any two means,there was st atistically significant between the differences in model group than that i n blank group,in glutamine low dose group and in glutamine high dose group at MDA level(P<0.05);There was statistically significant betweent he differences in glutamine low dose group than in glutamine high dose group at MDA level(P<0.05).Conclusion:Sepsis can lead to the occurrence of oxidative damage and oxidative stress in mice, Glutamine can improve the content of GSH-Px and the antioxidant enzyme SOD in mice, and improve the antioxidation ability.At the same time,Glutamine can reduce the content of MDA one lipid metabolite.Thereby, the use of glutamine can reduce toxic metabolite levels and oxidative stress damage,and so it can protect the organism. This study found that there is not statistically significant between the protective effect of Gln high dose group and Gln low-dose group on the oxidative damage in mice. |
PDF Full Text Request