Relationship Between The Serum Levls Of Interleukin-10 And Interleukin-10 Gene Promoter Polymorphisms And Hepatitis B Virus Infection In Guangxi Population

Objective To investigate expression of interleukin-10 (IL-10) in different clinical types of patientwith chronic hepatitis B virus(HBV) infection and to explore their clinical significience, to explore the correlation between single polymorphism(SNP) of IL-10 gene promoter-1082G/A and -592A/C and its clinical outcome of patients with HBV infection, and the correlation between SNP of IL-10 gene promoter-1082A/G and the expression of IL-10 in serums patient with HBV infection.Methods The patients chosen as the researched subject from the FirstAffiliated Hospital of Guangxi Medical University were 256 cases of ch-ronic HBV infection, including 59 liver cirrhosis (LC),151 chronic hepatictis B(CHB) and 46 chronic asymptomatic HBV carrier (ASC),as well as 52 control groups. Enzyme linked immuno sorbent assay(ELISA) was used to tested the serum levls of IL-10 and polymerase chain reaction-restriction fragment length polymorphism assay(PCR-RFLP) was used to detect SNP at IL-10-1082A/G and-592A/C. Fluorescent-quantitative PCR was used to tested the serum HBV-DNA copies.Results (1) The serum levls of IL-10 weresignificantly higher in LC patients than those in ASC patients and healthy controls(P<0.05) and there was no significant difference in the serum le-vls of IL-10 in LC patients and CHB patients(P>0.05).The Spearman rank correlation Correlation Analysis showed that IL-10 had a positive correlation to the level of HBVDNA replication (rs=0.476,P<0.05.(2)There was no significant difference in genotype AA,AC and CC frequencies of IL-10-592A/C in each chronicHBV group compared with healthy controls,as well as allele A and C frequencies(P>0.05).There was significant diff-erence in genotype GG,GA and AA frequencies of IL-10-1082G/A in ea- ch chronic HBVgroup compared with healthy controls, as well as allele Aand C frequencies (P<0.05). The genotype AA and allele A frequenciesof IL-10-1082G/A were significantly higher in LC patients than those in CHB patients,ASC patients and healthy controls(P< 0.05).No significant difference was found in frequencies of genotypes and alleles of IL-10 promoter -592 A/C and-1082G/A between the HBV-DN<1.0× 103copies/ml group and HBV-DNA> 1.0 X 103copies/mL group.The highest serum levls of IL-10 was genotype AA, followed by genotype GA and genotype GG (P<0.05).Conclusion (1)IL-10 plays a role in immune responses that may be involved in chronic HBV infected state.(2)IL-10 may play a role in inhibition of hepatic fibrosis in the disease progression of liver cirrhosis. (3) IL-10-1082G/A genotype GG and allele G mightbe associated with both susceptibility of HBV infection and the clinical types of patients withchronic hepatitis B in Guangxi population.(4)The SNPs of IL-10-1082G/A affect the level of IL-10 in serum,and the genotype AA lead to high expression of IL-10 and genotype GG lead to low expression of IL-10.