| ObjectiveTo explore the intervention effect of TRPV5 and TRPV6 in bone tissue of osteoporosis in ratsthe expression rule and the cog, and observe the clinical application effect of BushenQianggu, provide the basis for promoting the modernization of traditional Chinese medicine for the treatment of osteoporosis.Methods1. From six month old healthy female SD rats 50, weight (350+20) g, were randomly divided into normal control group, model group, the intervention of Cornus officinal is glycosides low dose group, the middle dose Cornus officinalis glycosides intervention group, the intervention of Cornus officinalis glycosides in high dose group,10 rats in each group. Except the normal control group, the other group the rats were ovariectomized osteoporosis rat model of experimental simulation. Cornus officinalis glycosides groups each given different doses of cornel total glycoside extract orally 1 times a day, the normal control group, model group were given equal volume of saline 1 times, a total of 12 weeks. At the end of the experiment the rats underwent dual energy X-ray bone density detection, right femurs for quantitative PCR to detect the expression of TRPV5/TRPV6 gene.2. Collection of clinical osteoporotic fractures and intertrochanteric hospitalized in the First Affiliated Hospital of Guangzhou University of Chinese Medicine for the treatment of the fracture in 24 cases, on the basis of patients’ wishes, were divided into control group and Xianlinggubao treatment group, respectively, before treatment began at 12 weeks follow-up of patients in the treatment of osteoporosis, application specific quality of life Chinese table from the symptoms of pain, physical function, ability of social adaptation, psychological function dimension score of each dimension of difference, comparing before and after treatment in patients with osteoporosis.ResultsThe experimental rat bone density of Cornus intervention groups were compared with the model group, the difference was statistically significant (P< 0.05). The dogwood intervention significantly improved the high dose, middle dose group, bone density, low dose group and the difference was statistically significant (P< 0.01). TRPV6 and TRPV5 were expressed in bone tissues of rats in each group, and the expression of TRPV5 gene in the model group was increased compared with the normal group, and the relative expression of TRPV6 and TRPV5 was decreased compared with the normal group. Under the intervention of Fructus Corni in bone tissue of the rats the TRPV5 and TRPV6 gene had different degree rise in the middle and low dosage, TRPV5 and TRPV6 gene with the dosage increased, its expression increased. High dose intervention, two groups of gene expression amount is dose intervention group decreased, but expressed genes from the two times than the relationship between, high dose group has was higher than that of the other groups of TRPV6, TRPV5 times than the relationship.Patients before and after treatment with the control group comparison, in pain and physical function difference was statistically significant (P< 0.01), in the social adaptation ability, mental functional dimension compared with no statistical significance (P> 0.05).ConelusionFructus Corni can promote the expression of bone tissue in ovariectomized rats of TRPV5 and TRPV6 channels, and through adjusting the relative expression quantity, thereby regulating bone broken bone cells and bone cell function, the osteoporosis rats bone metabolism balance to bone formation direction of change, maintain bone mass, improve postmenopausal osteoporosis. Cornus officinalis glycosides can significantly improve bone density in ovariectomized rats, prevent the occurrence of osteoporosis, deserve to do further research, exploitation and utilization. Femoral intertrochanteric fracture patients after application of anti osteoporosis treatment on the quality of life improve utility, but due to patients as people with social attributes, fracture rely solely on the drug treatment and can not completely solve the patients improve living conditions facing problems, in fracture treatment is still to be to encourage patients to take a positive attitude to face life, to help rebuild the confidence of patients to further improve the postoperative quality of life of the patients. |
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