| BackgroundGingival hyperplasia, which means the hyperplasia of gingiva due to gingival inflammation or medicine and other etiology reasons, is common in drug-influenced gingival hypertrophy or hereditary gingival fibromatosis caused by taking calcium ion channel blocker in hypertension and cardio-cerebrovascular diseases. Transglutaminases (TGs), a kind of multifunctional protein, makes the protein molecules form ε-(γ-glutamyl) lysine isopeptide bond or covalent binding of polyamine and histamine. Since its significant affections on post-transcriptional protein modification, TGs plays an important role in growth, differentiation and apoptosis of cells. Transglutaminases 2 (TG2) is a member of the TGs Family, a bounch of human diseases is known to be connected with the metabolic activities of extracellular matrix (ECM) that TG2 participates in, including fibrotic diseases, nervous system diseases, coliac diseases, diabetes, etc.According to several researches, TG2 has a tight connection with the attacks of diabetes and periodontal diseases, and has been a New Target in endocrine diseases treatment. Pathological feature of drug-induced gingival overgrowth and hereditary gingival fibromatosis is significant hyperplasia of connective tissues and collagen, which is related to intracellular calcium regulation. Since all the diseases above belong to fibrotic diseases of gingival tissue, TG2 might play an important role in pathological process of human gingival hyperplasia and might be seen as a test marker.ObjectiveThis experiment is to determine connection between TG2 and human gingival hyperplasia diseases, and to provide theoretical foundation for target drug-treatment of hyperplastic gingival diseases.MethodsHealthy and hyperplastic gingival speciments including drug-induced gingival hyperplasia and hereditary gingival fibromatosis were collected and paraffin sections prepared. HE staining and immunohistochemical staining were conducted to analyze the degree of hyperplasia of gingival tissue and the expression of TG2 in gingival tissue respectively.Results1.Histology results showed that compared with healthy gingiva, epithelium and connective tissue of hyperplastic gingival tissue were obviously thickening, collagen fibers were more coarse and arranged densely.2.The analysis results of immunohistochemistry showed that, contrasting with healthy gingiva, expression of TG2 in hyperplastic gingiva enhanced significantly and was more obvious in lamina propria.ConclusionThe results show that TG-2 probably plays an important role inpathological process of DGO and hereditary gingival fibromatosis. |
PDF Full Text Request