Anticancer Effect Of Xanthono-pyridine Derivative XP-15 On Human Lung Carcinoma A549 Cells

Objective: To investigate anticancer effect and potential mechanism of 2-morpholino-N-(7-oxo-7H-chromeno[3,2-h]quinolin-9-yl) acetamide(XP-15) on human lung carcinoma cell line A549 in vitro. Methods: Cell viability was measured by MTT assay and colony formation assay. PI/Hoechst33258 dying and flow cytometer were used for apoptosis analysis. Intercellular calcium concentration and mitochondrial membrane potential were detected by spectrofluorophotometer. A549 cells treated with XP-15 were collected for Bad and metallothionein 1A(MT-1A) transcript analysis by real-time reverse transcriptase–polymerase chain reaction(RT-PCR). Results: XP-15 inhibited cell proliferation of A549 cells in a dose-dependent manner and induced A549 cells apoptosis. After exposure to XP-15, intercellular calcium and mitochondrial membrane potential were decreased, MT-1A mRNA up-regulated in A549 cells. However, there were no differences in mRNA levels of Bad between XP-15 group and control group. Conclusion: XP-15 can induce apoptosis on A549 cells, which might be associated with decrease of intercellular calcium and mitochondrial membrane potential.