Expression Of MicroRNA-101, COX-2, TS In Different Survival Of Patients With Colorectal Cancer And Their Relationship

BackgroundColorectal cancer’s morbidity and mortality of our country has a rising trend, no decline and smooth running signs, only just 14 years the number have 63.4% been rose up. The average increase 4.87% every year[1]. The deformity trend have a delicate connection to the genetic factor, the environmental factor, the adjustment of the diet structure and the economic development mode of the population. There are a lot factors affect the treatment effect. With the development of economy, simple operation and use of intravenous drug chemotherapy has passed, recent years, a variety of advanced treatment methods take gospel to tumor patients and their families, but expensive treatment costs make tumor treatment emerged an awkward situation. More individualized treatment decision has become the focus of clinical research to improve the effect of clinical treatment. Individualized treatment need more accurate diagnostic methods, at present serological indicators have not been bring into the tumor staging among, because the serological marker of known is still relatively limited at present, and the relationship of the prognosis and cancer is not clear, it lead to this important subject have not been used. So we have to increase research effort of the serum tumor markers.ObjectiveExperiment 1:To investigate the correlation of cyclooxygenase-2(COX-2) and t hymidylate synthase(TS) expressions in Cancerous tissue with different disease free survival of patients with colorectal cancer.Experiment 2:To explore the relationship between the expression of both Micro RNA-101 and COX-2 in colorectal cancer and the relationship between disease free s urvival and patients’ MicroRNA-101.MethodsExperiment 1:1. Object:To follow-up colorectal cancer patients after curative resection, and rec ording DFS (disease free survival). Taking DFS as an important standard for screenin g,choosing 30 patients with DFS<48 months and 30 patients with DFS>48 months,a t otal of 60 patients, Department of Pathology providing the paraffin block of patients’c ancer tissue and adjacent normal tissues as the object of study.2. Methods:immunohistochemistry (SP), the cancer and adjacent tissue paraffin bl ock dewaxing, repair of TS and COX-2 antigens, using double antibody sandwich meth od catalyze the antigen combination with substrate, dyeing and reading under the light microscope, interpretation the expression level of TS and COX-2. compared the expr ession of Two groups’TS and COX-2, and do statistical analysis.3. Statistical methods:use non parametric X2 test to compare with the observ ed values of two groups’ expression of TS and COX-2.Experiment 2:1. study object:screening of resectioned colorectal cancer patients in our hospita 1, select 25 cases whose disease-free survival is less than 48 months as A group,25 cases whose disease free survival is more than 48 months as group B,25 cases ulce rative colitis patients with operation as group C.2 experimental methods:using fluorescent in situ hybridization method:using flu orescent dye labeled MicroRNA-101 probe, join it into the de waxed colorectal cancer tissue, normal tissues adjacent cancer, After hybridization,then using fluorescence m icroscopy to observe and record the results. the expression of COX-2 was detected by immunohistochemical method in colorectal cancer tissues, normal colon tissue and ulcerative lesions, and analysis the relationship between the patients’ disease free sur vivalin and COX-2.3. Statistical methods:Use K Independent Samples Test analysis the statistical d ifference of expression of MicroRNA-101 in different groups.Use Sperma correlation coefficient analyze whether the statistical difference about expression of MicroRNA-101 and COX-2 in different tissue.ResultsExperiment 1:1. In 50 patients with colorectal cancer, the positive expreesion rate of TS and COX-2 were 90.00% and 76.00%, respectivly.2. No significant difference was found in the expression of TS between the pa tients with different disease-free survival (P=0.646).3. The expression of COX-2 of patients whose DFS were longer than 48 mont hs were significantly lower than those whose DFS were shorter than 48 months(P=: 0.033).Experiment 2:1. MicroRNA-101 in Cancer tissue:group A (Mean Rank=32.24) positive rate w as the lowest, group B (Mean Rank=43.75)was the medium, group C (Mean Rank=58. 58) was the highest, there were significant differences between them(P<0.001). The e xpression level of groups A, B of patients with carcinoma were lower than normal tis sues(P<0.001).2. COX-2 in Cancer tissue:group A (Mean Rank=50.26) positive rate was the highest, group B (Mean Rank=35.78) was the lowest, group C (Mean Rank=49.13) c lose to the group A, there were significant differences between them(P=0.015). The ex pression level of groups A, B of patients with carcinoma were lower than normal tiss ue(P<0.001).3. there is a significant negative correlation between MicroRNA-101 and COX-2 in the cancer tissues and normal tissues; there is a significant positive correlatio n of MicroRNA-101 and COX-2 in ulcerative colitis lesions.Conclusion1.COX-2 was significantly related to the disease-free survival of patients with colorectal cancer, which might be a marker to predict the prognosis of colorectal ca ncer.2. colorectal cancer patients’ Disease free survival was significantly related t o MicroRNA-101 and COX-2, the longer survival time, the positive expression rate of MicroRNA-101 is higher,the positive expression rate of COX-2 is lower.3. In the cancer tissue and normal tissue the positive expression of COX-2 and MicroRNA-101 was negatively correlated, but in mucosal of lesions of patients with ulcerative colitis the relationship between these two markers are not obvious. This may be because the expression of MicroRNA-101 induced inhibition of COX-2, but i n ulcerative colitis disease have some reason blocking the inhibitory effect.Of course,th is still need further research.