Body Weight Supported Treadmill Training On Stem Cells Transplantation In Rats After Spinal Cord Injury Of Nerve Cells Differentiation Of Experimental Research

Objective:To investigate the effect of bone marrow stem cells (BMSCs) transplantation combined with body weight supported treadmill training on neural functional recovery of rats with spinal cord injury. Methods:Select the Sprague-Dawley male rats, imitate Allen method, and use the improved simple device to produce T11 complete spinal cord injury model. The final will be made in 40 SCI rats models successfully according to the different intervention methods. The rats were randomly divided into stem cell transplantation group, body weight support treadmill training group, combined treatment group (stem cell transplantation combined with partial body weight support treadmill training) and control group (no intervention treatment), and 10 rats in each group. After one week of the spinal cord injury operation, stem cell transplantation were performed on the rats in the two groups (the stem cell transplantation group and combined treatment group).When in the third passage BMSCs, before the 1st day post transplantation labeled with 5-ethynyl-2’-deoxyuridine (Edu), The rats in body weight support treadmill training group and combined treatment group were received body weight support treadmill training, and the other two groups for free activities. To determine motor function recovery through (Basso-Beattie-Bresnahan, BBB) movement function score after the 1st,2nd,3rd,4th, and 5th week of SCI. On the 5th of SCI, apply immunohistochemical staining and immunofluorescence staining to detect Neural specific markers:neuron specific enolase (NSE), microtubule associated protein (MAP-1(3), vimentin (VIM) positive expression, to observe the survival of the transplanted cells, differentiation of transplanted cells and lesion of nerve fiber recovery. Results:BBB motor function score displayed that combined treatment group BBB score was (6.60±0.97) on the 2nd week of SCI operation, which was significantly higher than the other 3 groups that were stem cell transplantation group and the body weight support treadmill training group, their BBB scores were respectively (5.00 ± 0.67) and (4.80 ± 0.63), and were higher than that of control group, but no statistical significance of BBB differences between the two groups (P>0.05). After third week, the stem cell transplantation group’s BBB score was (8.00±0.67), higher than that of body weight support treadmill training group BBB score (6.80±0.79), (P<0.05) with statistically significant difference. The imunohistochemistry shows there are different degrees of neural specific markers (NSE, MAP-1β, VIM) positive cells in rats by filling in the SCI organization, semi quantitative expression of spinal cord tissue in rats of each group to read in value by semi quantitative analysis method, combined treatment group were (4.72±0.19) points, (4.50±0.22), (4.62±0.27), was significantly higher than the other 3 groups (P<0.05), with significant difference. The immunofluorescence shows group Lneural specific protein markers (NSE、 MAP-1β、VIM) fluorescence expression significantly better than the rest of the group, and showed obvious nerve fiber proliferation and differentiation.Conclusions:Stem cell transplantation combined with partial body weight supported treadmill training can effectively promote the recovery of neural function of rats with spinal cord injury, and the effect is better than single stem cell transplantation group and the weight loss of ambulation training group.