The Effect Of 5-aza-CdR Enhances The Chemosensitivity Of Breast Cancer MCF-7 Cells To Paclitaxel And Epirubicin

Objective:In this experiment, through the DNA methylation inhibitor 5-aza-CdR interference of breast cancer lines MCF-7 cells in vitro proliferation, combined with the existing classical chemotherapy drug paclitaxel and epirubicin, discuss whether there exists between the two superimposed cumulative effect, provides the new way for the treatment of breast cancer chemotherapy.Methods:The MCF-7 cells were treated with drug(group A as blank control),with 5-aza-CdR(group B),5-aza-CdR combined with epirubicin(group C),5-aza-CdR combined with paclitaxel(group D),5-aza-CdR combined with paclitaxel and epirubicin(group E) and paclitaxel combinef with epirubicin(group F),respectively.MTT assay was used to determine the survive concentration of 80% in 24 h,48h and 72 h. Explore 5-aza-CdR, epirubicin and paclitaxel on breast cancer MCF- 7 cell experiment appropriate concentration and time.According to the experiment it is concluded that the appropriate concentration and time, drug intervention group in advance.The morphology of the cells was observed under microscope.Flow cytometry was used to examine the apoptosis rate.And the western- blot was used to determine the expression of PGP and MMP- 2.Results:1.Microscope:In 48 h,under microscope by 10 * 10. Nucleo cytoplasmic ratio and reduce the occurrence of cells in the experimental group, with inconspicuous nucleoli, kidney shaped and increase of performance, and there is a considerable part of the cell surface invaginations and shrinkage.2.Detecting Cell proliferation by MTT colorimetry: 5-aza-CdR combined with paclitaxel and epirubicin inhibitory effect on the proliferation of MCF-7 cells showed a dose and time dependent manner(P < 0.05), with the increase of 5-aza-CdR combined with paclitaxel and epirubicin concentration, the survival rate of MCF-7 cells was gradually reduced(P < 0.05), and 5-aza-CdR combined with paclitaxel and epirubicin in the same concentration of the inhibitory effect of prolonged MCF-7 cells growth rate also increased(P < 0.05).3.Detecting MCF-7 cells apoptosis by Annexin V FITC method: After 5-aza-CdR combined with paclitaxel and epirubicin(group E) effects MCF-7 for 48 h, The cells appeared apoptosis phenomenon, with the increase of concentrations of 5-aza-CdR combined with paclitaxel and epirubicin,MCF-7 cells showed apoptosis(17.11%±1.25%) increase proportionally(P<0.05). 4.west-bolt: On group E(5-aza-CdR combined with paclitaxel and epirubicin), the expression of PGP and MMP-2 are lower than other groups of five(P<0.05).Conclusion: 5-aza-DcR can promote cell apoptosis to inhibit MCF-7 cells proliferation of breast cancer cells in vitro.5-aza-DcR in vitro can down regulate PGP and MMP-2 expressing.5-aza-DcR in vitro enhances the chemosenstivity of paclitaxel and epirubicin in breast cancer MCF-7 cells.