| Objective:1. This study aimed to discuss the associations between diet, life factors and childhood obesity of 6-12 school-aged children through dietary survey.2. Based on the animal and human studies, we planned to study the physiological function of CCDC80 under different conditions. And to further analyse the association between CCDC80 and obesity and insulin resistance, so as to provide new theoretical basis and research directions for obesity and insulin resistance, as well as the prevention and treatment for diabetes.Methods:1. Children’s dietary behavior:We used two-stage sampling method on this cross-sectional survey. A self-administered questionnaire was distributed to 3515 children aged 6-12 years old of 11 primary schools in Hongkou District in Shanghai. The questionnaire was catergorized into four parts:basic information, food consumptionand eating behaviors. Eating behaviors includes: Breakfast information, milk and dairy products intake and snacks consumption.Informed consents were obtained from all participants’ parents.Children finished the questionnaire together with their parents. Multivariate regression models were used to explore the relationship between dietary and lifestyle behaviors and overweight and obesity of school-aged children.2. Animal and population study:Our research collaborated with Shanghai Biomodel to build CCDC80 gene knockout mouse model, to explore the key role of CCDC80 in obesity and insulin resistance and to further clarify its physiological function and mechanism. We collected both the visceral adipose tissue and the subcutaneous adipose tissue from the Hospital of Tangshan City, from who had a benign tumor.Adipose tissues were collected during elective abdominal surgery, and stored at -80℃. A self-designed questionnaire was used to collect information on demographic characteristics, including name, age, and sex, chronic disease history, smoking and drinking status; anthropometric indicators:height, weight, waist circumference, hip circumference, and blood pressure. We planned to explore CCDC80mRNA expression in different adipose tissues, and its correlation with obesity and insulin resistance.Results:1. Children’s dietary behavior:A total of 3515 students participated in the survey,2400 was of valid, boys 1210 (50.4%), girls 1190 (49.6%), and the response rate was 68%. The mean age was (9.26±1.27) years old. The study found that life factors of school-aged children were closely related to obesity. The prevalence of obesity of the paticipants was 10.9%,14.0% of boys, and and7.8% of girls. The combined incidence of overweight and obesity was 26.1%,35.1% of boys and 17.0% of girls. Study found that screen time is an independent risk factor for overweight and obesity. Multivariate analysis found that, compared with children who spent >3 hours/day on "screen time", those spent ≤2 hours/day or 2-3 hours/day had a lower obesity rate (AOR=0.34,95%CI: 0.20-0.60, P<0.01;AOR=0.41,95% CI:0.20-0.84, P=0.02).2.Animal model:The animal study found that CCDC80 gene knock mice was different from wild-type mice in glucose tolerance and insulin tolerance test. In the same glucose concentration stimulation test, the AUC of KO mice was slightly higher than wild-type mice. Further vitro glucose-stimulated insulin secretion test found that compared with the control group, islet cells of CCDC80 knockout mouse with low concentrations of glucose (2.5mmol/L) had their insulin peak secretion latency; while cultured with high concentrations of glucose (16.7 mmol/L)the peak and the lowest insulin secretion were lower than the control group.3. Population study found that within overweight and obesity group, their VAT CCDC80mRNA expression was lower than that in SAT (t=2.50,P=0.03). Further pearson correlation analysis found that VAT CCDC80 gene expression decreased along with their waist circumference (R=-0.47, P=0.04).Conclusions:1.In Shanghai, the overall overweight and obesity rate for school-aged children was about 26.1%,15.2% for overweight and 10.9% for obesity. Nearly a quarter of Shanghai school-aged children were overweight or obese, and rate has been rising over the past few decades. Time children spent on watching TV, playing games and using computersis closely related to the incidence of obesity. Based on this, further prevention interventions should focus on how to reduce children’s screen time and meditation behaviours to control the high incidence of obesity.2. Knockout CCDC80 gene might cause certain effects on mouse pancreatic islets function. KO mice pancreatic islets showed decreased insulin secretion level after glucose-stimulated in vitro. It turned out that CCDC80 might play a certain physiological role in insulin resistance.3. CCDC80mRNA expression level in visceral fat of overweight and obesity population was lower than the subcutaneous fat. And was negatively associated with waist circumference.This demonstrated that adipokine CCDC80 might involve in insulin metabolism and energy balance in the body, and was one of the protective factors of obesity and insulin resistance. |
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