| Aflatoxin B1(AFB1), is classified by the International Agency of Research on Cancer(IARC) as Group I human carcinogen. It could reduce immunosuppression causing by immune organs injury, which is usually related to the occurrence of cancer. Aloe is a traditional edible and medicinal plant containing variety of functions, such as antioxidant, immunomodulatory, antitumor and so on. However, the effect of aloe against AFB1-induced spleen injury has not been reported. This paper aims to investigate the intervention of aloe polysaccharides(AP) and aloin, two active ingredients in aloe, on subchronic AFB1-induced spleen injury in rats. At the same time, it will give economic advantage to aloe industry. This has positive significance for the prevention of AFB1, while has potential value to the development of aloe industry.64 male wistar rats(5 weeks old) were divided into eight groups based on body weight after adaptive feeding for one week(week 0): Control group; Control group with AP(60 mg·kg-1·bw·d-1); Control group with aloin(30 mg·kg-1·bw·d-1); AFB1 model group(200 μg·kg-1·bw·d-1); Low AP group(20 mg·kg-1·bw·d-1); High AP group(60 mg·kg-1·bw·d-1); Low aloin group(10 mg·kg-1·bw·d-1); High aloin group(30 mg·kg-1·bw·d-1). The first and second weeks, all rats were treated with appropriate amount of water(Control group and AFB1 model group) and corresponding doses AP and aloin according to body weight every day. From 3 to 7 weeks, the AFB1 model group and the intervention groups were daily gavaged to the 200 μg·kg-1·bw dose of AFB1 dissolved in corn oil, and the other three groups received the equal volume of corn oil.1 hour after, all rats were operated according to 1 and 2 weeks. Observed the growth state of the rats, measured the relevant indicators of immune function to assess the intervention of AP and aloin on AFB1-induced spleen injury, and then explored the possible mechanism from the oxidative stress status and cell apoptosis of spleen.The results showed that(1) it made the rats spiritual malaise, the average daily food intake decreased, body weight growing slowly by subchronic AFB1-treated. While AP and Aloin intervention could significantly improve the status.(2) AP and aloin could improve AFB1-induced immunosuppression of rats by improving immune organ index, inhibiting spleen tissue lesions, regulating T lymphocyte subsets improving cellular immune function by increasing CD3+, CD4+ T lymphocyte ratio, decreasing CD8+ ratio and increasing CD4+/CD8+ ratio, and inhibiting the expression of IL-1β, IL-6, TNF-α and IFN-γ.(3) Exploring the mechanism, AFB1 not only significantly induced the increase of ROS and MDA in spleen of rats, but also decreased the activity CAT, GSH, GSH-PX, SOD and the mitochondrial membrane potential of spleen to exacerbate the oxidative stress. But AP and aloin intervention could significantly improve the state of oxidative stress in AFB1-treated rats.(4) AFB1 resulted in activation of the mitochondrial pathway of apoptosis in spleen showed by flow cytometry and RT-PCR. However, AP and aloin could significantly improve the results by downregulating the expression of Bax and caspase-3 m RNA, upregulating the expression of Bcl-2 m RNA and Bcl-2/Bax ratio.Therefore, AP and aloin possess a significant preventive effect on the AFB1-induced spleen injury in rats, which may be related to their good antioxidant capacity and regulation on apoptosis. The present research provides theoretical guidance for the development and utilization of aloe. |
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