Polymorphism Of MICA/MICB Genes And Their Association With Acute Myeloblastic Leukemia M2 Type

Objective:(1)To study Acute myeloblastic leukemia M2 type serum levels of the patients.In order to analyze disease diagnosis and treatment monitoring clinical significance of s MICA in leukemia M2 type.(2)To study the distribution characteristics of the MICA/B genes in acute myeloblastic leukemia M2 type.Further discussion the polymorphism of MICA/B genes association with leukemia M2 type.Methods:(1)To detect s MICA serum leves sample of 64 patients in Acute myeloblastic leukemia M2 type By Elisa method.The correlation the serum s MICA gradually change during treatment progress.(2)MICA /B sequence alleles in 64 leukemia M2 type patients through PCR-SSP and PCR-SBT method, and the correlation were analyzed an the same time.Results:(1)The serum leves of s MICA by acute myeloblastic leukemia M2 type patients could increase(410.978±176.295)pg/ml.After 18 patients through DA chemotherapy drugs treatment turn into part relief,serum levels of s MICA(272.71±79.231)pg/ml,and it is significantly different from ones in control group(P<0.05).(2)12 MICA alleles were found in Acute myeloblastic leukemia M2 type,among which MICA*00801/02 was the dominant allele(28.2%).The others were MICA*010(22.7%).The rarest alleles were MICA*024(0.8%),MICA*022(0.8%).MICA allele frequencies were significantly different from ones in control group.Among the MICA-sequence alleles,the MICA*019 allele was more(OR:10.42,95%CI:1.1532-94.1390, Pc=0.0375),while the MICA*008allele was less(OR:0.5765,95%CI:0.3696-0.8993,Pc=0.0196),frequently observed in leukemic M2 type.(3)11 MICB alleles were found in Acute myeloblastic leukemia M2 type,among which MICB*005:01/02 was the dominant allele(35.2%).The others were MICB*009N(11.7%) and MICB*003(10.9%).The rarest alleles were MICB*006(0.8%).MICB allele frequencies were significantly different from ones in control group.The frequency of the MICB*00501/02 allele significantly lower than that in the control group(OR:0.3859,95%CI:0.2427-0.6134,Pc=0.0001).Conclusion :(1)Acute myeloblastic leukemia patients M2 type serum levels of the patients were increased,Diagnosis of figure specificity sensitivity AUC=0.953,s MICA have diagnostic value of M2 leukemia.(2)MICA * 019 allele is associated with acute myeloblastic leukemia M2 type there is a positive correlation. MICA*008 allele distribution frequency was increased in M2 type leukemia.MICA*008 allele is associated with acute myeloblastic leukemia type M2 negative correlation.(3)Between acute myeloblastic leukemia M2 type and MICB*00501/02 allele was negatively correlated.