Bmscs Transplantation Affect MCAO Rat Model Expressing MiR-34a And Survivin Protein

Objective:To investigate the BMSCs transplantation’s function of anti-apoptosis and nerve rehabilitation, we established cerebral ischemia-reperfusion model with modified Longa suture emboli, and then transplanted BMSCs via tail intravenous injection. Finally we evaluated the neurological deficits,detected the expressional level of mi R-34 a and survivin protein at different time points.Method:1.Establishment of the rat model: Selecting the healthy male SD rats whose weight was between 250~300g, then applied the modified Longa suture emboli to establish cerebral ischemia-reperfusion rat model.2.The judgement of successful model:On the basis of 5 score method, who scored 1-3 points was a successful rat model.Using TTC staining to verify infarction size.3.Groups of the Experiment: A total rats who match the requirements of experiment were randomly divided into 4 groups: sham control group,operation group,PBS transplantation group, MSCs transplantation group.The MSCs transplantation group and PBS transplantation group were separately injected 1ml(containing l ×l06)MSCs and PBS via tail intravenous injection after the rat model successfully established.4.Corresponding indicators’ detection:Evaluated the neurological deficits by adopting the Modified Neurological Severity Score. Immunohistochemical detection and Quantitative real-time PCR detection was employed to detect the expressional level of survivin protein and mi R-34 a.Result:1.On the basis of 5 score method,the successful rate of modules set was 72.22%.Finally, 54.17% of the whole rats were used to corresponding indicators detection.2.Modified Neurological Severity Score(m NSS) result :At 12 h,1d,3d,7d time points,sham control group: 0±0.00;operation group:9.83±2.04,15.17±1.47,12.17±2.23,10.83±1.47;PBS transplantation group:9.67 ± 1.97,15.00 ± 1.67,12.00 ±2.10,11.00 ± 2.37;MSCs transplantation group:10.00 ± 2.53,13.33 ±2.16,8.33±1.21,6.83±0.75.It showed that the m NSS scores of operation group,PBS transplantation group,MSCs transplantation group were significantly higher than sham control group(P<0.01), operation group and PBS transplantation group showed no significant difference(P>0.05),while comparing MSCs transplantation group and operation group,PBS transplantation group, the m NSS scores showed no significant difference in 12 hour(12h) and 1 day(1d), whereas significantly higher in 3 day(3d) and 7 day(7d).3.Survivin positive cells(%):At 12 h,1d,3d,7d time points,sham control group: 0 ± 0.00;operation group:11.00 ±2.61,19.17 ± 3.76,31.00 ± 3.03,13.00 ± 2.61;PBS transplantation group:10.67 ± 2.16,20.00 ± 3.46,32.33 ± 4.18,14.33 ± 3.14;MSCs transplantation group:16.33 ± 2.16,29.00 ± 5.48,44.83 ± 3.76,25.00 ±4.60.It showed that the positive cells of operation group,PBS transplantation group,MSCs transplantation group were significantly higher than group A(P<0.01), operation group and PBS transplantation group showed no significant difference(P>0.05), MSCs transplantation group were significantly higher than operation group and PBS transplantation group(P<0.05).4.the expressing level of mi R-34a: At 12 h,1d,3d,7d time points,sham control group: 1.1187±0.1241;operation group:4.7698 ± 0.2569,3.7305 ± 0.2985,3.0697 ± 0.2067,2.6898 ±0.1998;PBS transplantation group:4.7388 ± 0.2147,3.7705 ±0.2378,2.9782 ± 0.2153,2.6682 ± 0.1879;MSCs transplantation group:4.0532±0.2082,2.8338±0.2391,2.5525±0.1101,2.0438±0.2608. It showed that the mi R-34a’s expressing level of operation group,PBS transplantation group,MSCs transplantation group were significantly higher than sham control group(P<0.01), operation group and PBS transplantation group showed no significant difference(P>0.05), MSCs transplantation group were significantly lower than operation group and PBS transplantation group(P<0.01).Conclusion:1.BMSCs transplantation can reduce neural deficits induced by cerebral ischemia-reperfusion, which performed as a protective factor in ischemia brain injury.2.The ischemia-reperfusion injury could up-regulate the expression of mi R-34 a in rats brain;BMSCs transplantation may probably play a role in anti-apoptosis by down-regulating the level of mi R-34 a, then up-regulating the expression of survivin protein indirectly.