| Biomedical Materials are a kind of biomaterials with the functions of disease diagnosis and treatment or tissues and organs replacement and repair. Hyaluronic acid (HA) is a polymer which is widely present in humanbody. HA has excellent viscoelasticity and biocompatibility. Poly-aspartic acid (PASP) is a synthetic biopolymer, whose main chain will be broke down into non-toxic and harmless natural amino acids during the degradation process.To improve the mechanical property of the hyaluronic acid-polyaspartic acid hydrazide hydrogel (HAALD-PAHy hydrogel), we added glutaraldehyde (GN) and Genipin (GN) into the HAALD-PAHy gel system. Then we got the hydrogels modified by glutaraldehyde (HAALD-PAHy-GA) and hydrogels modified by genipin (HAALD-PAHy-GN). Thereafter, crosslinked hydrogels were characterized by gel content, swelling ratio,rheological and thermal properties. Furthermore, the crosslinking degree of the hydrogels can be controlled by adjusting the concentration of GA or GN. Our results showed that the by adding GA or GN will increase the gel content. Scanning electronic microscopy (SEM) showed that the hydrogel exhibited smaller porous. The rheological analysis revealed that the storage modulus (G’) of the hydrogel was3752Pa and4859Pa, respectively.Subsequently, stimulation and sensitization experiments were done and the results proved the good biocompatibility of the hydrogel. Finally, this hydrogel system was injected into rabbit’s lacrimal ductules to investigate its effect of embolization. It took sodium fluorescein about20min to get from rabbit’s eyes to its nasal cavity at14days after the injection. These results suggest that our hydrogels can become promising material for lacrimal plugs. |
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