| Objective: Gastric cancer is a common digestive system tumor. Gastric cancer has ahigh morbidity and mortality in the all types tumor. Currently, the early diagnosis of gastriccancer is the only way to achieve good prognosis of gastric cancer. Currently, the earlyscreening of gastric cancer mainly depends on the gastroscopy. The gastroscope biopsy andpathological examination is very limited. And the technical depended on the proficiency ofphysicians and pathology doctor’s judgment. Therefore, the development of new cancer of thestomach is very important for the prevention and control of the early diagnosis for gastriccancer. MicroRNAs (miRNAs) is a kind of length about20~25nucleotides of non-codingsmall RNA. MicroRNA, regulates the expression of about1/3the genes. In recent years,they found that MicroRNAs almost exist all tumor cells and participant in the occurrence oftumors, transfer and infiltration. Compared with the normal tissue, the expression of miR-21and miR-155are very high in the tissue of gastric cancer. Besides, the expression of miR-155is associated with the transfer of gastric cancer. On the contrary, the expression of miRNA-148in gastric cancer tissue is lower than the normal control group. At present, miR-21, miR-15and miR-148in serum was used as a measurement of the early diagnosis of gastriccancer. Unluckily, the miRNA in the serum failed to locate the type of the tumor.. In order toimprove the early diagnostic rate of gastric cancer and accuracy, clinical gastroscopecombined with the expression of miRNA were used to diagnose the early gastric cancer.Methods: We first collect the samples sampling specimens of gastric mucosa and judgethe morphology of astric mucosal lesions under gastroscope. The sample were divided intofour group according to the tissue pathology, including gastric cancer group, polyps group,superficial gastritis group, poor ly differentiated adenocarcinoma group. At the same time, theexpression of miRNA-21, miR–155and miRNA-148in the tissue were detected byqRT-PCRResults: The expression of miR–21and miR-155in the collected gastric cancer groupwere significant higher than in the gastritis group, indicating that the morphology under thegastroscopy and the expression of miRNA in the tissue is consistent. However, the expression of miRNA-21in the tissue of one case was significantly higher than that of the normal group.In contrast, Cases of micrornas-21express one group was obviously higher than that ofnormal group, but few cancer cell was found in the sample under the pathologicalexamination and gastroscopy, indicating that the mirRNA marker could add the rate ofdiagnose of gastric cancer. In add ition, the expression level of miR-155in gastric cancertissues was associated with the staging of gastric cancer. But the expression of miRNA–21and miR-155levels has nothing to do with patient’s age.Conclusion: Combination of gastroscope morphological examination and miRNAmarker screening could improve the rate of diagnosis of gastric cancer. |
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