Effects Of Electro-acupuncture Treatment On Expression Of AQP3in Cartilage Cells Of Rabbit With Knee Osteoarthritis

objective: Osteoarthritis (OA) is a chronic disease on bone and joint,with characters of progressive articular cartilage degeneration, destruction andosteophyte formation, which occurs in large weight-bearing joints, such asthe knee, hip, spine and distal interphalangeal joints. Clinical symptoms arejoint pain, limited mobility and joint deformities and so on. Knee OA is themost common site of occurrence, the study found that aquaporin3(AQP3)can promote the development of knee OA, AQP3expression in patients withknee OA cartilage cells was significantly higher than normal articularchondrocytes. Knee OA as a progressive degenerative disease of the elderly,there is no complete cure, mainly taken the surgical and non-surgical treatmentto slow the progression of the disease, while EA is a non-surgical treatmentwith better clinical efficacy. Clinical observation of the current electricacupuncture knee OA more, but its intervention as a means to study themechanism of animal experiments has been relatively rare. Therefore, thisstudy using plaster cast immobilization method extensor bit fixed six weeksmaking rabbit model of knee OA, EA four weeks of treatment interventions, toinvestigate the effect of electroacupuncture on rabbit knee OA articularcartilage development and expression as well as AQP3by regulating theexpression of AQP3to interfere with the development of the feasibility of knee OA for the treatment of knee OA looking for new targets. Methods:(1)pre-experiment: Luzhou Medical College Experimental Animal CenterProcurement five healthy New Zealand white rabbits of either sex, weight2.3-3.2kg, after taking the right knee with plaster casts in the knee extensionposition, fixed range plane from the groin to the ankle1.5cm3cm place.Braking six weeks after the scalpel cut the medial femoral condyle and tibialplateau cartilage, hematoxylin-eosin staining. The results cartilage cell disorder,hyperplasia, thinning of the cartilage surface, there are fissures producecartilage tissue fibrosis, cartilage and synovial swelling, inflammatory cellinfiltration, synovial hyperplasia. Pathological complex with osteoarthritis,indicating successful modeling.(2) participants and interventions: LuzhouMedical College Experimental Animal Center again buy health36NewZealand white rabbits of either sex, weighing2.3-3.2kg, using a randomnumber table36rabbits were randomly divided into control group, modelgroup and the experimental group, each with12. The control group receivedno treatment, model group and EA group were modeling, six weeks afterremoving plaster casts. After modeling the model group makeselectro-acupuncture, electro-acupuncture treatment, while only group EA tiedwith the same posture in the treatment of stage; electro-acupuncture groupbegan to pick up the next day to give EA plaster treatment. Take xuehaixue,dubixue, yinlingquanxue, Yanglingquanxue, Zusanlixue, liangqiuxue six points,three groups received acupuncture instrument output line, the output frequency is sparse wave of2Hz, the needle20min,1day, continuous treatment14days.After the end of the experiment, using all the rabbits were sacrificed bycervical dislocation, observe the changes related indicators. Observation2indicators:①modified Mankin’s score according to the standard grossmorphology of the knee cartilage after the blank control group, model group,electro-acupuncture group3rabbits using the right method to quantify visuallyscore;②scalpel cut blank control group, model group, electro-acupuncturegroup three rabbits right knee medial femoral condyle cartilage, after10%neutral formalin fixed, paraffin embedded and sliced again for HE staining, inaccordance with the modified Mankin’s score standard form cartilage cellsusing optical microscopy methods to quantify the results of the observationscore;③enzyme-linked immunosorbent assay (ELISA) to detect fluid in theknee AQP3expression: people with anti-rabbit AQP3of ELISA reagentsAQP3expression was detected after the collection box rabbit right knee.(3)Statistical analysis: SPSS17.0statistical software for statistical analysis. Theresulting measurement data results using±s, using the t test to compare ratesof rabbit chondrocytes and cartilage structure score, HE staining score andscore integrity tide line to P <0.05was considered statistically significant.Results:(1) cartilage gross morphology: no knee joint effusion blank controlgroup of12rabbits Right, no inflammatory cell infiltration, cartilage, smoothsurface, no cracks, bluish-white, color depth and bright. Improved cartilagegross morphology of Mankin’s score of0points. Significant knee joint effusion and right after the model group, the cartilage surface roughness,cracks, gloss reduced inflammatory cell infiltration, cartilage surface grayishyellow, some appear erosions and ulcers; electro-acupuncture group and modelgroup situation similar but lesser degree of damage, according to a modifiedMankin’s score in the cartilage structure, a significant difference (P <0.01)differences.(2) HE staining pathological observation: the control groupshowing the position of the articular cartilage cells arranged in neat rows, thesame cell size, the full tide line; consistent layer thickness cartilage, cartilagesurface is flat and tidy; presence of cartilage defect model group layer, layer ofcartilage thinning, cartilage cells decreased significantly, a large cluster of cells,the tide line disturbances; electro-acupuncture group articular cartilage surfacewithout significant loss, cartilage cells arranged in neat compared with modelgroup, compared with an increase in the number of model group, comparedwith the model group thickness thickening visible tide line disorders. Theimprovement of the control group and model group, model group and EAgroup of Mankin’s score were compared, the differences were statisticallysignificant (P <0.05).(3) Measurement ELISA, Immunology AQP3expression:3expression was detected in both groups AQP3, but AQP3expression inmodel group was significantly higher than the control group and EA group (P<0.05). Conclusions:(1) Rabbit model of knee osteoarthritis knee extensionplaster cast immobilization method is successful.(2) AQP3increasedexpression in articular cartilage of knee OA.(3) EA can effectively relieve the symptoms of knee OA, and its mechanism of action may be related to theregulation of AQP3by reducing the water content of transport, reduce thedegradation of extracellular matrix, reducing damage to the articular cartilage,thus easing the processes related to knee OA.