| ObjectiveMoxibustion is a form of traditional Chinese medicine that has been practiced for centuries. Despite its long history and worldwide application, the biological mechanisms of moxibustion in relieving pain have been poorly defined. In this experiment, to establish that ’Zusanli(ST36)’ point has the local anti-nociceptive effects of moxibustion in SD rats; to find that whether ATP mediates the anti-nociceptive effects of moxibustion by evaluating the effects of moxibustion on inflammatory pain in SD rats.MethodsSD female rats (200±20g) were used in all experiments. Peripheral inflammation was induced by injection of CFA (mixed with an equal amount of oil, total volume0.1ml,sigma) in the plantar surface of the right hind paw of rat. ATPase and ARL67156was administered in the Zusanli point before acupuncture. For moxibustion was placed vertically on the distance of lcm the top of Zusanli point located3-4mm below and lateral1-2mm for the midline of the knee. Mechanical allodynia was evaluated using repeated stimugations with a Von Frey filament exerting2g of force onto the plantar surface. The percentage of negative responses of a total of ten trials was calcugated for each foot. To avoid conditioning to stimugation, we interposed a5s rest period. Behavioral parameters were evaluated before intraplantar injection of CFA (that is, day0), on day4in rat receiving the CFA injection, and after moxibustion, injection of ATPase and ARL67156(that is, measure for2h,30min once), unless otherwise noted.Resugts1. Administration of the moxibustion in the ipsilateral Zusanli point (ST36) evoked increase in the threshold to touch in the CFA+Moxibustion group.The pain threshold improved from2.85±0.23g to8.27±0.47g (P<0.01, Tukey-Kramer). But it is not significant change in the CFA group, the pain threshold improved from2.83±0.32g to2.855±0.28g (P>0.05, Tukey-Kramer). Similarly, the pain threshold is not significant change in the control group and moxibustion group. At30min after moxibustion, the pain threshold is the most obvious improve,79.93%of the baseline and the moxibustion analgesic effect continued1hour. In addition, At2h after moxibustion, this effect was disappeared.2. In the CFA+Moxibustion+ARL67156group, administration of ARL-67156in the right Zusanli point (ST36) evoked a sharp increase in the threshold to touch pain, the pain threshold improved from2.30±0.18g to10.66±0.64g(P<0.01, Tukey-Kramer) and it is not significant change in the CFA+ARL67156group, the pain threshold improved from2.08±0.19g to2.08±0.17g (P>0.05, Tukey-Kramer). For the degree of pain relief, The CFA+Moxibustion+ARL67156group is better than CFA+Moxibustion group, it improves2.39±0.55g(P<0.01, Tukey-Kramer). In addition, the other groups are not significant change. At60min after moxibustion, the pain threshold is the most obvious improve,96.86%of the baseline (P<0.01, Tukey-Kramer) and at30min to90min, the moxibustion analgesic effect is better than CFA+Moxibustion group(P<0.01, Tukey-Kramer). In addition,2h after moxibustion, this effect was disappeared. So we can consider that ARL67156wougd enhance the beneficial effects of moxibustion.3. In the CFA+Moxibustion+ATPase group, administration of ATPase in the right Zusanli point (ST36) didn’t increase in the touch pain threshold, the pain threshold improved from1.86±0.13g to1.98±0.15g (P>0.05, Tukey-Kramer) and in the CFA+ATPase group, the pain threshold improved from2.09±0.21g to2.23±0.28g (P>0.05, Tukey-Kramer). For the degree of pain relief, the CFA+Moxibustion group is better than CFA+Moxibustion+ATPase group, it improves2.39±0.55g(P<0.01, Tukey-Kramer). And the other groups are not significant change. At each time point, the moxibustion analgesic effect is lower than CFA+Moxibustion group(P<0.01, Tukey-Kramer). In addition,2h after moxibustion, this effect was disappeared. So we can consider that ATPase wougd inhibit the effects of moxibustion.Conclusions1.Moxibustion has a good analgesic effect in chronic inflammatory pain model;2.Acceleration of ATP metabolism inhibition moxibustion analgesic effect;3.Manipugation of ATP metabolism enhance moxibustion analgesic effect. |
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