| With the improvement of people’s living standard and the change of the diet, the prevalence of diseases such as metabolic syndrome (MS) and type2diabetes increased year by year. And the related complications had caused serious damage to human body health. Recently, a large number of researches have shown that L-arabinose has a lot of biological activities including lowing blood glucose levels, improving the glucose tolerance, depressing blood fat levels, reducing blood press levels, easing liver tissue damage of type2diabetes rat, reducing the fat accumulation of visceral organs and delaying weight growth. It is widely used in a variety of feilds such as food industry, medicine and chemical industry. In this paper, the metabolic syndrome rats were used as experimental models, and this paper studied the intervention effect of L-arabinose on glucolipid metabolism and the preliminary mechanism in metabolic syndrome rats through observation of the state and liver pathological sections of rats, determinations of related physiological and biochemical indexes(including body weight, fast blood glucose, blood lipids, microartery systolic blood pressure, serum insulin) and measurements of the gene expression quantity at the molecular level.Firstly, metabolic syndrome rat models were established by feeding the rats with high sugar-fat-salt. The feeding time was14weeks. The success rate on establishment of metabolic syndrome rats model were evaluated by checked the indicators, including body weightt, fast blood glucose, blood lipids, microartery systolic blood pressure, serum insulin. The results showed that the prevalence of obesity, hypertriglyceridemia, low HDL-C concentration, hypertension and hyperglycemia in rats model induced by high sugar-fat-salt diet was82.8%,68.8%,16.7%,93.8%,54.2%. Finally, standard metabolic syndrome model rats were selected, the success rate of metabolic syndrome rats model was at a rate of72.4%.To investigate the effect of L-arabinose on modulating the glycolipid metabolism in rats with metabolic syndrome, the selected experimental rats were divided to4groups:MS control group, L-arabinose high dosage group (525mg/(kg-bw)), L-arabinose medium dosage group (175mg/(kg-bw)), L-arabinose low dosage group (87.5mg/(kg-bw)). Then the control group was divided to two groups randomly, normal control group and L-arabinose control group. The invention effects of L-arabinose on physiological and biochemical indexes (including body weight, fast blood glucose, blood lipids, microartery systolic blood pressure, serum insulin, free fatty acids and HOMA-IR) were studied by gavaged with different dosages of L-arabinose (87.5mg/(kg·bw),175mg/(kg·bw),525mg/(kg·bw)) to the metabolic syndrome rats. The results showed that the rats weight gain had a slowing trend after given L-arabinose. After L-arabinose treatment, level of microartery systolic blood pressure in3dosages groups decreased obviously, the difference had statistical significance (P<0.01). Meanwhile the level of fast blood glucose also decreased significantly (P<0.05).The liver morphology changes of experimental rats were observed by tissue paraffin section. The effects of L-arabinose on inflammation of metabolic syndrome were measured; results indicated that L-arabinose could markly improved inflammatory state in metabolic syndrome rats.To study the molecular mechanism of L-arabinose on metabolic syndrome, the gene expression of CPT-1α, ACCa and PDK4in liver were detected by polymerase chain reaction. The changes of mRNA were used to explore whether L-arabinose participated in regulating the expression of these genes. Results showed that L-arabinose could protect rats exposed to high nutritious diet through up-regulated expressions of mRNA for hepatic CPT-1α, PDK4and down-regulated expressions of mRNA for hepatic ACCa. |
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