The Regulation Of FADD Expression On Apoptosis Pathway Of T Cell Apoptosis In Patiens With Idiopathic Thrombocytopenic Purpura

Object:Immune thrombocytopenic purpura is an autoimmune disease, theabnormality function of B lymphocyte on the pathogenesis of ITP havedetermined in recent years. Recent studies demonstrated that the abnormalfunction of T lymphocytes also existed in the pathogenesis of ITP. Through thegene expression of FADD, Fas, FasL, Caspase-8, FLIP，Foxp3+and the levelof IL-10, TGF-beta in peripheral blood T lymphocyte of ITP, we observe thechanges of the T cell apoptosis pathway and Treg cells function in patients withITP.Methods:1, the separation peripheral blood T lymphocyte.2, the level of FADD, Caspase-8, FLIP, Fas, FasL, Foxp3+and beta-actinmRNA was detected by RT-PCR.3, the level of plasma IL-10and TGF-beta was detected by ELISA.Results:1, the expression of peripheral blood T cells FADD mRNA：the ITP groupwas0.129±0.039, the control group was0.159±0.025, ITP group expressionwas significantly lower than control group,the difference has statisticssignificance(P<0.05).2, the expression of peripheral blood T cells Fas mRNA：the ITP group was0.127±0.051, the control group was0.202±0.040, ITP group expression wassignificantly lower than control group,the difference has statistics significance （P＜0.01）.3，The expression of peripheral blood T cells FasL mRNA：the ITP group was0.255±0.059, the control group was0.213±0.059, ITP group expression wassignificantly higher than control group,the difference has statistics significance（P＜0.05）.4，the expression of peripheral blood T cells Caspase-8mRNA：the ITP groupwas0.271±0.077, the control group was0.378±0.098, ITP group expressionwas significantly lower than control group,the difference has statisticssignificance（P＜0.01）.5, the expression of peripheral blood T cells FLIP mRNA：the ITP group was0.208±0.041, the control group was0.141±0.048, ITP group expression wassignificantly higher than control group,the difference has statistics significance（P＜0.01）.6，the expression of peripheral blood T cells Foxp3+mRNA：the ITP group was0.042±0.008pg/ml, the control group was0.099±0.011pg/ml, ITP groupexpression was significantly lower than control group,the difference hasstatistics significance（P＜0.05）.7, the plasma IL-10in ITP group was30.03±12.11pg/ml, the control groupwas40.91±15.25pg/ml, the level of ITP group was significantly lower thancontrol group,the difference has statistics significance（P＜0.05）.8，the level of the plasma TGF-β in ITP group was3211.62±1348.22pg/ml, thecontrol group was4763.85±1919.09pg/ml, the level of ITP group wassignificantly lower than control group,the difference has statistics significance（P＜0.05）.Conclusion:1, FADD mRNA expression of T lymphocytes in ITP patients. 2, there are abnormal expression of upstream molecules Fas and FasL anddownstream molecules Caspase-8mRNA also found in apoptosis pathway ofFADD with ITP in the peripheral blood T lymphocyte.3, the expression of apoptosis inhibiting protein FLIP in T lymphocytes withITP, could further inhibit apoptosis signaling pathways.4, T lymphocyte immune function on Treg cells in patients with ITP wassignificantly inhibited.