Methods Based On Metabonomics To Study The Syndrome Of Spleen Deficiency And Dampness Stagnation Cancer Biomarkers

Objective:To find related biomarkers during occurrence and development of spleen deficiency and dampness stagnation syndrome-colorectal cancer using ultra high perform ance liquid chromatography-quadrupole-time of flight-mass spectrometry (UPLC-Q-TOF-MS) based metabonomics approach.Methods:According to the basic diagnosis methods of TCM syndrome differentiation and diagnosis criteria of the subjects were divided into normal group, spleen deficiencyan d dampness stagnation and deficiency of Yin toxin colorectal cancer group(specific different iation standard appendix CRF table), the subjects were divided into normal group and spl een deficiency and dampness stagnation syndrome-colorectal cancer group. The plasma a nd urine samples were analyzed by UPLC-QTOF-MS, all the data containing the retention t ime, ion intensity and exact mass were imported in the MassLynxTM software for partial le ast squares discriminant analysis (PLS-DA). Then, score plots were used to analyse the overall distribution and aggregation state of two group samples, and S-plots were used t o find potential biomarker most contribute to the difference between two groups. In add itiona, these metabolites in plasma and urine were identified based on the retention time, pr ecise molecular mass and fragment ions of the compound reported in the literatures. Finally, the spleen deficiency and dampness stagnation syndrome-colorectal cancer related bioma rker were found through characterizing related metabolic pathways in spleen deficiency an d dampness stagnation syndrome-colorectal cancer patients.Ressults:Some spleen deficiency and dampness stagnation syndrome-colorectal canc er related biomarker were found by UPLC-Q-TOF-MS based metabonomics approach, su ch as glycine, L-serine, choline, arginine, citric acid in plasma and alanine, citric acid, p henyl acetyl glutamine in urine.Conclusion:The diagnosis of the disease of the large intestine and the differential diag-nosis of the damp stagnation of spleen qi and the syndrome of the dampness stagnation of the spleen qi and the syndrome provide the objective and the quantitative standard.