| The first part: Clinical ResearchObjective:Through the use of infant diarrhea treatment of antibiotic-associated diarrhea particles were observed before and after treatment in children with abdominal pain or discomfort, changes in stool frequency, stool and other symptoms of diarrhea in infants and evaluate the treatment of antibiotic-associated diarrhea particles of clinical efficacy.Methods:Random grouping method, using SPSS software will be included in the standard case 66 cases, were randomly divided into treatment group(33 cases(fall off in 2 cases, rejecting 1 case) and control group 33 cases fall off(3 cases), treatment group taking baby diarrhea granule in treatment, the control group taking Clostridium butyricum powder(Chang Lekang) treatment, treatment group known as the baby diarrhea granule group, the control group called the changlekang group, the two groups after 1 weeks. Symptoms, signs were observed and recorded before and after the treatment of antibiotic associated diarrhea and improve adverse reaction.Results:After 1 weeks of treatment, the two groups of patients with clinical symptoms and signs were improved obviously,(1) The clinical symptoms of the treatment group the total effective rate was 96.7%, the control group was 86.7%, the efficiency of two groups had no significant difference.(P>0.05). The cure rate of treatment group was 70%, 40% in the control group, the two groups had statistical significance(P<0.05) compared to the cure rate.(2) the total scores of symptoms before and after treatment: the total symptom score after treatment in the two groups were significantly decreased compared with before treatment(P<0.05), the treatment group after treatment the total symptom score decreased than that of control group after treatment, the total score(P<0.05).(3) the main symptoms integral comparison before and after treatment: the main symptoms of the two groups after treatment were significantly decreased compared with before treatment score(P<0.01), the treatment group after treatment compared with the control group after treatment score decreased significantly(P<0.05),(4) compared the secondary symptom score before and after treatment: two groups after treatment were lower than that of the secondary symptom score before treatment was significantly decreased(P<0.01), the treatment group after treatment, secondary symptoms compared with the control group in terms of complexion and perianal redness two symptoms had no significant difference(P>0.05), and discomfort, bloating, in the spirit of thirst symptoms had significant difference(P<0.05).(5) two groups of adverse reaction and safety assessment of treatment: the treatment group 30 cases and control group 30 patients had no any adverse reaction. The basic situation of the two groups before and after treatment of heart rate, respiration, including temperature did not change significantly, indicating that the treatment group and the control group had no obvious toxic and side effect.Conclusion:The clinical study showed that treatment group, has the remarkable curative effect and application of granule in the treatment of antibiotic associated diarrhea in infants. Its curative effect is better than that of changlekang in control group, the results from a clinical perspective to verify the effectiveness of antibiotic associated diarrhea in infant diarrhea granule in the treatment and feasibility, Chinese medicine has obvious advantages in the treatment of this disease, and a further research and discussion of the value.The second part: Experimental StudyObjective:By observing the relevant indexes after treatment of antibiotic-associated construction and animal models, and explore the mechanism of infant diarrhea particle therapy AAD provide experimental evidence for clinical research.Methods:Choose a healthy 4-week-old Kunming mice SPF level 50, male and female, weight(13 ~ 15) g. Male and female, cage adaptive feeding six days after the adoption of digital control group were randomly divided into nine, making the module 41, the model group mice were fed with antibiotics modeling method, After 4 days of modeling, 8 randomly selected control group and model group 8, by pulling the tail and abdominal massage, sterile collect fresh mouse droppings. Sterile fresh faeces c ollected from each mouse 0.1 g, diluted ploidy, and placed in incubator flora. Pathological changes in line with flora successful modeling tips. Randomly as normal group and model group mice were 1, were sacrificed by cervical dislocation, colon tissue of mice was taken for pathological observation. The remaining mice were again made module 40, were randomly divided into five groups, namely, model group, Chang Le Kang group, infant diarrhea particles high dose group, infant diarrhea particle dose group, low- dose group particles infant diarrhea. N = 8, male and female, are allotted to cages and fed drug intervention, the normal group and blank model group were given equal volume of normal saline, Chang Le Kang Chang Le Kang suspension were given orally, infant diarrhea particles were given high infant diarrhea particles in suspension fed low doses. One week after gavage colon contents sterile collection, testing mice flora situation.Results:(1) the general observation: model group mice showed reduced activity, poor appetite, will dilute; the mice in treatment groups were improved in different level of the performance. Model group mice weight were reduced after treatment before making model, the model of mouse body weight increased, close to the normal weight group.(2) the results of quantitative detection of each group of intestinal flora after modeling: model group mice intestinal bacteria Bifidobacterium, Lactobacillus, Enterococcus enteric bacilli was significantly decreased, p<0.05 increased, compared with the normal group.(3) the results of quantitative detection of intestinal flora after treatment in each group: compared with changlekang group and infant diarrhea granules high, low group and the model group, the intestinal flora were significantly increased, p<0.05. infant diarrhea group is better than that of particles, and the infant diarrhea changlekang group, middle dose group was superior to the infant diarrhea granules granules in high dose group. Compared with changlekang group and infant diarrhea, low dose group p>0.05, no significant difference between the two.(4) Intestinal mucosa histopathological observation: the model group mice intestinal mucosa was superficial injury and inflammation; infant diarrhea granules high, low dose group and the changlekang group pathological changes of intestinal mucosa were improved, the infant diarrhea particles dose group improved more obviously, microscope was used to observe the repair of intestinal mucosa was significantly better than the changlekang group.Conclusion:The experimental results show that antibiotics fed mice can cause intestinal disorders diarrhea, and infant diarrhea particles can improve the symptoms of diarrhea. The mechanism may be to protect the intestinal mucosa, in the balance of gut bacteria related. |
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