The Establish Of Naringin Etal Six Functional Components In Online RP-HPLC Methods And Antioxidation Research In Different Citrus

Citrus fruits, there is a variety of natural bioactive components in it, which are the active ingredients of the body has a certain disease prevention and control actio n, so the more active ingredients have been used in the food industry and the phar maceutical industry, etc. It is also the recent citrus fruits are many reasons for scien tists of great concern. This paper focuses on the effectiveness of the many compone nts of citrus fruits and their antioxidant research, providing a theoretical reference fo r future research and development scientists citrus fruit. This paper mainly studies th e establishment and antioxidant capacity of six kinds of citrus fruit RP-HPLC ingred ients, to provide a theoretical reference for the future research and development of c itrus fruits of scientific workers. Using the method of RP-HPLC, the effects of six c omponents in different varieties of citrus fruits Naringin, Hesperidin, Naringenin, Hes peretin, Limonoids, Nomilin) and11kinds of polyphenols component content determ ination; Reference GB/T19416-2003method. The GB/T5009.157-2003method, resp ectively, by colorimetric method、RP-HPLC method, the contents of flavonoids in dif ferent citrus fruit samples, the organic acid(malic acid, tartaric acid, citric acid, succi nic acid)content analysis; Using ORAC method, antioxidant research on the different varieties of citrus fruits, the method azo compound2,2’-azobis(2-methylpropionam-id ine) dihydrochloride(AAPH)as peracetic source of free radicals, fluorescein sodium so dium Fluorescein(FL)is a fluorescent indicator to evaluate the antioxidant capacity of the antioxidant microplate fluorescence analyzer for analysis, the results of the wate r-soluble vitamin E analogue trolox equivalent to express.Results are following:1Naringin, Hesperidin, Naringenin, Hesperetin, Limonoids, Nomilin in the range of1.7～266.8μg/mL good linear relationship, R2>0.9993, six kinds of functional components The percent of recovery was94.6%-111.9%, the limit of quantification range0.40ng-4.77ng, detection limits range0.12ng-1.43ng. The method is accurate, reliable, and can be used in the six kinds of functional components in citrus fruit content of samples was measured, Among them, the content of Naringin in citrus fruits was higher than the rest of citrus samples, determination of Naringin in South Africa produced by red grapefruit reaches1598.7mg/kg, the content of Hesperidin in orange samples higher than other samples, the content of hesperidin in Gannan navel orange of up to3302.4mg/kg.2The eleven symmetry and separation of functional components of a good seed polyphenols, can be separated within75min completely.within a good linear relationship, R2>0.9999.The eleven kinds of phenols RP-HPLC method for the determination of the content of different citrus fruit samples, and achieved satisfactory results, In citrus fruit, the majority of pomelo fruit polyphenol species is slightly lower than the rest of the citrus samples, such as the Guangxi kumquat produced of Hyperoside content up to308.4mg/kg.3Total flavonoids (as rutin) quality of R2=0.9952in the range of0.2mg-lmg, the linear relationship is good, the organic acid detection data, tartaric acid, malic acid, citric acid, succinic acid in the range of11.1～1001.0μg/mL good linear relationship, the square of R were more than0.9999.The GB/T19416-2003method、the GB/T5009.157-2003method for the content of total flavonoids in citrus fruit, different samples of organic acids (tartaric acid、malic acid、citric acid、succinic acid) content detection, different citrus fruit samples show different content difference, The content of citric acid and citric fruits were higher than those of the other samples of citrus lemon, South Africa up to11618.6mg/kg. The ORAC method, Trolox standard in the range of3.125μM～50μM good linear relationship, R2=0.9967, standards、controls Vc、samples with RSD%<20%, there is the maximum value of ORAC in the pummelo of Jiangxi province, up to58.1μmol Trolox TE/g.4The ORAC method for antioxidant activity of different citrus fruits, access to accurate results, Among them, the ORAC value of pomelo fruits are slightly highe r than the rest of the citrus samples, average ORAC values is31.62±16.03μmol T rolox TE/g.