| Objective:Pummelo peel(Mao Juhong)is the Geoherbs of Guangdong HuaZhou.It is rich in flavonoids ingredients,which has the function of antioxidative effect,and is worthy of in-depth research and development,and the use of specialty resources.Therefore,the author has carried out the screening of Mao Juhong varieties and the research on the development and efficacy of Mao Juhong’s antioxidant products,so as to provide scientific basis for the deep development of the Guangdong specialty resources.Method:1.Use high performance liquid chromatography(HPLC)method to determine the content of Mao Juhong’s naringin,rhoifolin,naringenin and apigenin of different varieties.HPLC method was used to study the fingerprints of different varieties of Mao Juhong.Using Stata software to cluster analysis of the 21 batch of the fingerprint of Mao Juhong.Determine the content of flavonoids in different varieties of Mao Juhong and the ability of scavenging DPPH free radical in vitro.Preliminary study on difference of the content of flavonoids in different varieties of Mao Juhong and the scavenging ability of DPPH.2.Use water extraction and alcohol precipitation method to extract the total flavonoids in Pteris species Mao Juhong active ingredient.Determination of different rootstock species DPPH radical scavenging of the Mao Juhong Pteris bud medicinal ability by DPPH method.The inclusion technology will citri grandis total flavone solution of prepared inclusion liquid.Add honey,apple acid,xylitol and other accessories to regulate the taste flavor of Mao Juhong liquid.Add 10 g/L chitosan solution as oral liquid clarification agent.Add the amount of 8%of total volume of solution,then cold storage standing 24 h and filtered,and the filtrate is used full automatic filling and sealing machine sealing,and sterilizing and leak detection.3.The Mao Juhong oral liquid was evaluated the ability of scavenging free radicals in vitro by DPPH method;Benzene liver lipid peroxidation of bromine generation mice gastric high,medium and low(3.0,6.0,9.0 mg.Kg-1)hair orange of three groups of dose oral liquid,30 days after the use of kit mice used in the determination of SOD and gsh-px in serum and liver of two kinds of antioxidant enzyme activity,a preliminary evaluation of the Mao Ju Hong liquid antioxidant capacity.Results:1.Respectively the contents of naringin in the Pteris(Feng Wei),Jia Xiyang,Jin Qiandu,Huang Rongguo,Da Chaling,Mi Yejinmao,Guang Qing are:93.03 mg/g,121.26 mg/g,99.51mg/g,110.13 mg/g,101.43 mg/g,107.22 mg/g,66.28mg/g.Respectively the contents of Rhoifolin in the Pteris(Feng Wei),Jia Xiyang,Jin Qiandu,Huang Rongguo,Da Chaling,Mi Yejinmao,Guang Qing are:2.04 mg/g,4.35mg/g,2.31mg/g,4.13mg/g,4.04 mg/g,2.97 mg/g,0.71 mg/g.Respectively the contents of naringenin in the Pteris(Feng Wei),Jia Xiyang,Jin Qiandu,Huang Rongguo,Da Chaling,Mi Yejinmao,Guang Qing are:0.57 mg/g,0.91 mg/g,0.69 mg/g,0.80 mg/g,0.85 mg/g,0.63 mg/g,0.37 mg/g.Respectively the contents of apigenin in the Pteris(Feng Wei),Jia Xiyang,Jin Qiandu,Huang Rongguo,Da Chaling,Mi Yejinmao,Guang Qing are:0.325 mg/g、0.236 mg/g、0.308 mg/g、0.327 mg/g、0.261 mg/g、0.316 mg/g、0.156 mg/g.Removal of DPPH free radical clearance SA%respectively:14.3,22.6,14.8,21.8,20.2,15.9,20.2.Fingerprint study:the similarity of 21 group of different cultivars,orange medicine fingerprint was more than 0.90,there were 10 common peak,the first peak of naringin and the second peak of wild sumac glycosides.2.The results show that the Pteris with local pomelo grafting contents of wild sumac Pteris on the two varieties are above 0.5%,and their ability of scavenging free radicals in vitro is very strong,SA%were higher than 20.0.Pteris plant after bitter orange and pomelo variety root stock grafting,wild sumac contents were reduced to below 0.2%,their ability of scavenging free radicals in vitro SA%less than 11.0.Mao Juhong oral liquid 100 mL formula is that:the Mao Juhong flavone solution fluid inclusion(25 mg/mL)20 mL,80%honey 20 mL,malic acid,3 g,2 g,xylitol,purified water 60 mL.Add 8 mL of the chitosan solution concentration of 10 g/L and let stand for filtration,the oral liquid of naringin content is 50 mg/mL.3.1 mg/mL naringin clearing DPPH free radical clearance SA(%)of 51.55 on average.In the normal control group mice serum SOD,gsh-px activity of antioxidant enzymes are:5744.19 ± 73.73,73.73 ± 9.78,lavage Mao Juhong experiment of high dose group of mice serum SOD,gsh-px activity of antioxidant enzymes are:6289.88 ± 279.39,279.39 ± 39.28.The computed Stata/SE 11.0 statistics software,all have significant difference significance,P<0.01;Model group mice in the liver SOD,gsh-px activity of antioxidant enzymes were 66.40 ± 176.42,6.87 ± 7.07,lavage Mao Juhong among experiment of high dose group of small liver SOD,gsh-px activity of antioxidant enzymes are:81.11 ± 5.35,5.35 ± 10.15.The computed Stata/SE 11.0 statistics software,all have significant difference significance,P<0.01).Conclusion:1.Different batches of Exocarpium fingerprint and fingerprint similarity control shall not be less than 0.90.Contrast group of 21 different varieties of Mao Juhong active ingredient content were analyzed.Comprehensive evaluation is the high content of active ingredients selected Pteris Mao Juhong varieties.By means of different varieties in pummelo peel contains determination of effective components and its ability to remove DPPH free radicals,optimizing the Pteris varieties as the experimental materials medicine.2.Use Water extract-alcohol precipitation method to extract total flavonoids in Mao Juhong,and the method is simple and reliable.Grafting the scion can significantly influence the choice of Mao Juhong wild herbs sumac glycosides content and the ability of scavenging free radicals,should choose the local shaddock as Mao Juhong bud grafting of the root stock.Use honey,malic acid,xylitol solution of total flavonoids of taste masking,taste sweet and sour moderate;Use of chitosan as clarifying agent,applied to the preparation of wool orange oral liquid product.3.Mao Juhong oral liquid has a certain ability to remove free radicals in vitro;Appropriate doses of Mao Juhong oral liquid can improve the benzene bromide generation model SOD and gsh-px in serum and liver of mice two kinds of the activity of antioxidant enzymes,shows that the oral liquid has a certain antioxidant effect. |
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