| Background:Chronic pharyngitis is chronic inflammation occurred in pharyngeal mucosa, submucosal and lymphoid tissue. It’s hard to be cured. In recent years, air pollution and abuse of using antibiotic aggravate the case, and protracted course of disease. It’s known to all that the chronic pharyngitis are related to causing pathogenic bacteria, but more research found that disorder of the dysbacteriosis is more important causing factor. Therefore, use the microecological modulator properly and adjust flora balance in pharyngeal is much more important for preventing and curing this disease. If we want to adjust flora balance, the priority thing is analyze the changing characteristic of the flora in pharyngeal. The new technologies and methods such as DGGE and second- generation sequencing laid a foundation for that. And it’s a new way for effectively preventing and curing chronic pharyngitis by analyze the changing characteristic of chronic pharyngitis.Purposes:Analyze the microbial community structure between patience and health people. Find the differences of bacterial flora distribution who suffered chronic pharyngitis. Laid a foundation for guiding the doctor to adjust flora in clinical application use and targeted treatment.Methods:1. Find 15 people who naturally get the chronic sphagitis as the research group, find 15 health people as the contrast.2. Draw Peripheral Venous Blood from two group. examine the total number of white blood cell and the total number of other blood cell, contract them with these two group.3. Extract secreta by using germfree swab. To get to the pharyngeal secretion of extracted DNA and its joint degeneration after PCR amplification gradient gel electrophoresis(PCR- DGGE) method for flora diversity analysis. For DGGE electrophoresis results after comparing, the differences between two groups have rubber cutting strips of sequencing, the sequencing results Blast analysis in GenBank database, ultimately some differences in the two groups of bacteria species can be obtained.4. Streptococcus, Acinetobacter, staphylococcus aureus and staphylococcus epidermidis, ues this four species’ s Specific primers as comfirmatory PCR to identify whether their throat has the four species or not and their quantity differences.Results:1.In contrast with the health people’s samples, The total number of white blood cells in peripheral blood and the number of neutrophils difference was significantly increased.(p<0.05)2.The total number of Flora species in patience ’ s throat was significantly increased.(p<0.01)3.The sequencing results of these two group who has bacterial genus are: the specificity of the patients with chronic sore throat bacteria genera are: Gemella, veillonella, prevotella. Healthy specific bacteria genera are: Neisseria. In the bacterial genus that both group shared, Pseudomonas is relatively less in the patience group. Streptococcus they got more.4.PCR revealed that both the patience group and health group got the Pharyngeal streptococcus, but the total quantity of patience group’s Pharyngeal streptococcus is higher than health group(p<0.05). the quantity of acinetobacter and staphylococcus aureus is slightly higher than health group. I didn’t found staphylococcus epidermidis in either of the group.Conclusion:1.In patience’s Pharyngeal mucosa I found inflammatory response.2.Patience’ bacterial diversity is higher than health people, accompanied with dysbacteriosis.3.The features of dysbacteriosis in people ’ s throat who suffered chronic pharyngitis are: Facultative diplococcus, Veillonella, Prevotella, Streptococcus, Staphylococcus bacteria, increasing Acinetobacter and diminishing Pseudomonas. |
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