Expression Characteristics Of PTEN And NDRG1 In Colorectal Carcinoma And Their Prognostic Value In Colorectal Carcinoma Patients

[Objective] PTEN and NDRG1 is a important factor in the development of tumor. To investigate the expression and clinical significance of PTEN, NDRG1 in colorectal carcinoma and to evaluate their prognostic value in colorectal carcinoma patients.[Methods] The expressions of PTEN, NDRG1 were detected at their protein levels by immunohistochemical (IHC) staining and image analysis technique (IAT) in 21 cases of colorectal normal mucosa,30 cases of colorectal adenomas,91 cases of colorectal cancers.54 cases of colorectal carcinoma were followed to ascertain the influence of on survival of PTEN and NDRG1 patients with colorectal carcinoma.[Results] 1. The expressions of PTEN proteins in colorectal normal mucosa, colorectal adenomas, colorectal cancers were 90.48%,70.00%、54.95% respectively, indicating a gradual decrease during the development of colorectal carcinoma (P<0.05). There existed a differences between the expressions of PTEN proteins in colorectal adenocarcinoma with no regional lymph node metastasis and in those with regional lymph node metastasis and the correlation was negative (0.01<P<0.05, rs’=-0.240). There was no significantly differences between its expression and the degree of differentiation of the colorectal adenocarcinoma (P >0.05) and those patient’s gender, age (P>0.05)2. The expressions of NDRG1 proteins in colorectal normal mucosa,colorectal adenomas, colorectal cancers were 57.14%、63.33%、76.92% respectively, indicating a gradual increase during the development of colorectal carcinoma (P< 0.05). There existed a differences between the expressions of NDRG1 proteins in colorectal adenocarcinoma with no regional lymph node metastasis and in those with regional lymph node metastasis and the correlation was negative (0.01<P<0.05, rs’= 0.218). There was no significantly differences between its expression and the degree of differentiation of the colorectal adenocarcinoma (P > 0.05) and those patient’s gender (P> 0.05), but there was significantly differences between those patient’s age (P<0.05).3. The expression of PTEN and NDRG1 proteins in the colorectal adenocarcinoma was negative correlation relationship (P<0.05, rs’=-0.251).4. Prognosis of patient was affected by expression of PTEN proteins in colorectal cancers.5. Epidemiological diagnostic tests shows:detecting the expressions of PTEN in colorectal carcinoma combined with NDRG1, the sensitivity was 74.07% and the specificity was 52.38%, accuracy is 70.54%, positive predictive value was 88.89%, negative predictive value was 28.21%.[Conclusions] 1. Loss expression of PTEN protein may be an important molecular marker predicting the occurrence of colorectal carcinoma, and PTEN may be useful as a prognosistic marker in colorectal carcinoma.2. Expressions of NDRG1 proteins play a role during the process of development of colorectal carcinoma, but NDRG1 proteins can’t be a predicator of colorectal carcinoma.3. The auxiliary diagnosis of difficult cases can be used by combined detection of PTEN and NDRGl.