Clinical And Experimental Study On The BGR/ABL-negative Myeloproliferative Neoplasms By Adding Rhubarb Zhechong Pill

1.Objective:Through clinical observation and vitro assay,the auter analyses and probes into the curative effect and mechanism of Adding rhubarb Zhechong Pill curing the BCR/ABL-negative Myeloproliferative neoplasms,for traditional chinese medicine curing the the BCR/ABL-negative Myeloproliferative neoplasms provide the clinical curative effect and theoretical basis support.2.Methods: This research includes clinical observation and vitro assay two parts.(1)Clinical observation:A retrospective analysis of 45 patients with complete treatment data records for more than 5 months of our department BCR / ABL-negative myeloproliferative neoplasms patients, divided into three groups:â‘ polycythemia vera 12 cases; â‘¡ essential thrombocythemia 20 cases; â‘¢ idiopathicmyelofibrosis 13 cases. Chinese medicine treatment were given rhubarb Zhechong Pill,and supplemented by Western symptomatic and supportive treatment in all patients with serious illness. Specifically:rhubarb Zhechong Pill,on this basis, with the addition and subtraction cards continued to take him for the medical changed to the same prescription made Chinese medicine Miwan of drug taking in January after each pill 9g each time a pill, 2 times a day. Record changes in blood of patients with improvement of symptoms, three groups of patients before and after treatment were compared before and after treatment, and the effects were compared between the three groups.(2)Vitro assay:Preparation of rhubarb Zhechong pill containing serum, MTT assay proliferation of HEL cells, cell colony formation influence on cell colony formation assay, testing on apoptosis and cell cycle with Annexin V FITC / PI method.3.Results:(1)Clinical observation:The PV group after treatment, the number of red blood cells, hemoglobin, hematocrit compared with before treatment, there are significant differences, and white blood cells, platelets no significant difference. ET group after treatment, platelets, white blood cell count decreased significantly compared with the previous, the statistical significant difference, and no significant difference in hemoglobin before treatment. Hemoglobin compared with the previous the IMF group patients after treatment significantly increased, the statistical significant difference, and white blood cells and platelet count have no significant difference in the test. Three groups before and after treatment symptom scores compared were significantly different. Efficacy: PV patients markedly effective in 7cases, 3cases improved and ineffective in 2,the total effective rate was 83.3%. ET patients markedly effective in 12 cases,4 cases improved and ineffective in 4,the total effective rate was 80%.IMF patients markedly effective in 4 cases, 6 cases improved and ineffective in 3, the total effective rate was 76.9%.Effective rate in the PV group> ET group> IMF group,between the three groups efficacy of pairwise comparisons, statistical tests was no significant difference.(2)Vitro assay:Rhubarb Zhechong pill containing serum can significantly inhibit the proliferation of HEL cells in a dose and time-dependent manner; can inhibit the formation of HEL cell clones; HEL cells can induce apoptosis and cell cycle arrest in G0/G1 phase in a concentration-dependent manner.4.Conclusion:Through the clinical and experimental studies confirmed that rhubarb Zhechong pill may be a good medicine in treating BCR / ABL-negative myeloproliferative neoplasms. It is possible to improve PV, ET, IMF clinical treatment of patients,and it is worth further study.