Clinical Study And Experim Ental Research On Decreasing Ovarian Reservation By Guishen Pill

Objective With the change in people’s way of life and the accelerated pace of life, mental pressure increases, the incidence rate of decline of ovarian reserve is increasing, and the age is younger.Chinese Medicine for the treatment of decreasing ovarian reservation, recovery of ovarian function has better curative effect and unique advantage.This study on the base of the previous research, the basic pathogenesis of traditional Chinese medicine of tonifying the kidney to kidney deficiency, taking the theory as the instruction, adopts Guishen Pill in the treatment of diminished ovarian reserve,and to explore the possible mechanism of treating DOR by Guishen Pill through animal experiment, which aims to provide further scientific basis for Chinese medicine treatment of DOR, provide more thinking and clinical experience in the treatment. Methods ①This study selected in 2013 March to 2014 November were enrolled from Hubei Provincial Traditional Chinese Medical Hospital gynecology clinic 44 cases as the research object,with the western medicine diagnosis standard decreasing ovarian reservation and TCM syndrome differentiation type genus of kidney deficiency type standard,by Guishen Pill,continuous take 14 days for a course of treatment, a total of 3 months of treatment.Using self controlledstudy before and after treatment,comparise and analysis before treatment and after 3 menstrual cycles of TCM symptom score,total score, menstrual improvement and serum sex hormone examination results. ② To further confirm the therapeutic effect in the treatment of DOR by Guishen Pill,animal experiments conducted further research,to observe the effect of Guishen Pill on decreasing ovarian reserve mouse generally, estrous cycle, ovarian index, and the number of primordial follicles in vivo reproductive hormones, observed the effect of Guishen Pill on ovarian reserve function decline of ovarian function in mice,to investigate the possible mechanism of Guishen Pill prevent ovarian function decline.Results(1)Clinical observation:①The Guishen Pill treatment, among them 5 cases were cured,10 cases markedly effective, effective 21 cases,invalid 4 cases, the total effective rate was 90%,total effective rate was 92.5% for regulating menstruation; ②After the treatment of the patients with total score decreased significantly,lumbosacral pain, dizziness,tinnitus,hot flashes, insomnia, insomnia and dreaminess,Shenpi fatigue,loss of libido and clinical symptoms were improved greatly;③The Guishen Pill in the treatment of patients with FSH,LH,and FSH/LH ratio decreased significantly than that before treatment.④The blood routine, stool routine electrocardiogram, liver function detection were no significant after treatment,also no patients experienced nausea andvomiting and other adverse reactions.(2)Animal experiment:①After medication, groups of mice were also gradually improve, eating, gradually returned to normal, the hair is soft luster.Each treatment group mice weight compared with the model group increased, and the difference is statistically significant(P<0.05);the Guishen Pill in each dose group with no significant difference between bujiale groups(P>0.05).Guishen Pill in all dose groups showed no significant difference(P>0.05). ② Compared with the normal group,model group mice ovarian index decreased statistical significantly(P<0.05),Each treatment group had no obvious change(P>0.05);The treatment group compared with the model group,the model group mice ovarian index decreased, the difference was statistically significantthe(P<0.05).Guishen Pill in each dose group with no significant difference between bujiale groups(P>0.05).Guishen Pill in all dose groups showed no significant difference(P>0.05).Each index of uterus of mice did not see obvious change(P>0.05).③Modeling and treatment, in addition to Guishen Pill low dose group, the treatment group compared with the normal group there was no significant difference between the cycle(P>0.05);The treatment group compared with the model group,the cycle was shortened(P<0.05);Cycle of Guishen Pill low dose group and progynova group, Guishen Pill in middle and high dose group significantly increased,the difference was significant(P<0.05); There was not significant difference between the Guishen Pill,in the high dose group and progynova group(P>0.05).④After the model number of primordial follicles in ovarian mice in each group were significantly reduced compared with the normal group,with statistical difference(P<0.05);Compared with Guishen Pill low dose group and model group, primordial follicles was increased, but the difference was not statistically significant(P>0.05).Comparison of the treatment group and the model group, the number of primordial follicles were increased significantly, with statistical difference(P<0.05);No significant changes between the Guishen Pill each dose group and bujiale group(P>0.05). ⑤ Modeling and administration, compared with the normal group, model group AMH levels of Guishen Pill low dose group,the difference was statistically significant(P<0.05).The rest of the group,the AMH level showed no significant difference(P>0.05);Compared with the model group, the treatment group FSH decreased,AMH increased, the difference had statistical difference(P<0.05);No significant changes between the Guishen Pill each dose group and bujiale group(P>0.05);Guishen Pill low dose group AMH value was lower than that of Guishen Pill in the high dose group,the difference had statistical difference(P<0.05).Conclusion Method of Guishen Pill by nourishing kidney essence, which has good effect in improving the lumbosacral pain, hot flashes, insomnia, loss of libido and other clinical symptoms, at the same time reduce the value of FSH, AMH increased the level of effect is remarkable,and without any adverse reaction.Animal studies show that Guishen Pill can improve AMH level,decrease the level of FSH, increase the number of primordial follicles in DOR mice,we hypothesized that Guishen Pill may be through regulation of primordial follicles start,raising and dormant primordial follicles to maintain growth,so as to achieve the recovery of ovarian reserve function.Guishen Pill is the safe and effective treatment of ovarian reserve function decline.It’s worthy of clinical popularization and application.