Sports Fatigue Rats Hippocampal Cells Apoptosis PI3K/AKT Signaling Pathway

Objective: To observe the hippocampus tissue apoptosis in exercise-induced fatigue rats situation,the content of 5-HT, DA, P-PI3 K, AKT, P-GSK-3 and the changes of beta Ser protein level and PI3 K, AKT, P-GSK-3 gene expression, Analysis of the influence of exercise fatigue on the PI3K/AKT signal pathway in hippocampus of rats, to further explore the neurological biological mechanism of the central fatigue. Methods: 30 healthy male SD rats were randomly divided into two groups, respectively: model group(15 rats) and control group(15 rats). Start speed is 15 m/min, gradually increased to the highest speed is 35m/min; each time for about 20 min, the training period was about 7 weeks. The seventh week and the week before the same but the last exercise until exhaustion date. Immediately the abdominal aorta and 24 hours from rat hippocampus. Test index in blood HB, serum CK, BUN and T in the. In hippocampus 5-HT, DA content and PI3 K, AKT, GSK-3 beta protein and gene expression level.Results:(1) compared with the model group and the control group, HB, T content in the model group significantly decreased(P < 0.05). The content of CK, BUN increased significantly(P < 0.05).(2) compared with the model group with the control group, the content of 5-HT/DA in the model group increased significantly(P < 0.05).(3) compared with the model group with the control group, model group, cell apoptosis rate increased significantly in the model group(P < 0.05).(4) compared with the model group with the control group, model group PI3 K, AKT, GSK-3 beta protein expression increased significantly(P < 0.05).(5) compared with the model group with the control group, model group PI3 K, AKT, GSK-3 beta m RNA expression increased significantly(P < 0.05). Conclusion: High intensity exercise on hippocampal tissue in rats of PI3 K, AKT, GSK-3 beta protein expression increased.The expression of hippocampus PI3 K, AKT, m RNA significantly increased GSK-3 beta. Therefore, high intensity exercise can activate PI3K/AKT signaling pathway play an important role in anti apoptosis.