Expression Of IQGAP1、Rac1 And Cdc42 In Basal-like Breast Carcinoma And Its Clinical Significance

Objective:To detect the expression of IQGAP1、Rac1 and Cdc42 in basal-like breast carcinoma(BLBC)、 non-basal-like breast carcinoma(NON-BLBC) and normal breast tissue, to analyze the relationship of IQGAP1、Rac1 and Cdc42 with the clinicopathologic features of BLBC and the correlation between them and to explore the function of IQGAP1、Rac1 and Cdc42 in occurrence,development,invasion and metastasis of BLBC.Methods:Immunohistochemistry(IHC) was used to detect the expression of IQGAP1、Rac1 and Cdc42 in 60 cases of basal-like breast cancer, 45 cases of non-basal-like breast cancer and 35 cases of normal breast tissues. The data of Immunohistochemistry were analyzed by using SPSS 17.0 for windows,Using the ?2 test and Fisher exact test more positive to analyze the expression of IQGAP1、Rac1 and Cdc42 in BLBC、 NON-BLBC and normal breast tissues and o analyze the relationship of IQGAP1、Rac1 and Cdc42 with the clinicopathologic features of BLBC, α = 0.05 as inspection standard, P < 0.05 for differences with a statistical significance;Using the Spearman correlation analysis to analyze the correlation between IQGAP1、Rac1 and Cdc42,α = 0.05 as inspection standard, P < 0.05 for differences with a statistical significance;Using the partition of ?2 method to analyze the expression of two groups between BLBC、NON-BLBC and normal breast tissues, α = 0.05 as inspection standard, P < 0.0125 for differences with a statistical significance.Results:The expressions of IQGAP1 were 85.00%(51/60)、82.22%(37/45)、28.57%(10/35) in basal-like breast cancer, non-basal-like breast cancer and normal breast tissues, and the difference was statistically significant(P <0.05); the expression of IQGAP1 in BLBC was significantly higher than that in normal breast tissue, and the difference was statistically significant(P<0.0125);the expression of IQGAP1 in NON-BLBC was significantly higher than that in normal breast tissue, and the difference was statistically significant(P<0.0125);the expression of IQGAP1 positively correlated with tumor size and p TNM staging of BLBC(P <0.05). The expressions of Rac1 were 81.67%(49/60)、51.11%(23/45)、20.00%(7/35) in basal-like breast cancer, non-basal-like breast cancer and normal breast tissues,and the difference was statistically significant(P <0.05); the expression of Rac1 in BLBC was significantly higher than that in NON-BLBC, and the difference was statistically significant(P<0.0125);the expression of Rac1 in BLBC was significantly higher than that in normal breast tissue, and the difference was statistically significant( P < 0.0125);the expression of Rac1 in NON-BLBC were significantly higher than that in normal breast tissue, and the difference was statistically significant(P<0.0125); the expression of Rac1 positively correlated with pathological grade、lymph nodes metastasis and p TNM staging of BLBC(P <0.05). The expressions of Cdc42 were 85.00%(51/60)、55.55%(25/45)、31.42%(11/35) in basal-like breast cancer, non-basal-like breast cancer and normal breast tissues, and the difference was statistically significant(P <0.05); the expression of Cdc42 in BLBC was significantly higher than that in normal breast tissue, and the difference was statistically significant(P<0.0125);the expression of Cdc42 in BLBC was significantly higher than that in NON-BLBC, and the difference was statistically significant(P<0.0125);the expression of Cdc42 positively correlated with tumor size lymph nodes metastasis and p TNM staging of BLBC(P <0.05). There was positive correlation relationship between the expression of Rac1 and IQGAP1 in BLBC(r=0.404, P <0.05), there was positive correlation relationship between the expression of Cdc42 and IQGAP1 in BLBC(r=0.608, P<0.001), there was positive correlation relationship between the expression of Rac1 and Cdc42 in BLBC(r=0.645, P <0.001).Conclusions:IQGAP1、Rac1 and Cdc42 were over-expressed in BLBC,and the expression of IQGAP1 、 Rac1 and Cdc42 positively correlated with pathological grade 、 lymph nodes metastasis and p TNM staging of BLBC,indicating IQGAP1、Rac1 and Cdc42 were crucial importance in local invasion and distant metastasis of BLBC,and may play a key role in development and progression of BLBC,suggesting IQGAP1 、 Rac1 and Cdc42 can be used as BLBC indicators of poor prognosis and therapeutic targets in future.The expression of IQGAP1、Rac1 and Cdc42 were all relationship in BLBC,suggesting IQGAP1、Rac1 and Cdc42 participated in together the stage of tumor cell transformation and invasion in BLBC, suggesting to detecting the expression of IQGAP1、Rac1 and Cdc42 in BLBC may indicate the metastasis potential and prognosis in BLBC.