Exploratory Experiment Of Building The Atrial Fibrillation Model Of Rabbits By Telemetry Technology And Optimization Based On Experimental Animal Welfare

Introduction:Objective: To explore to build a kind of atrial fibrillation model of rabbits on the basis of wireless telemetering and simulation technology. Method:An implantable telemetering simulator which was independently researched and developed was hypodermically implanted in New Zealand rabbits. The implantable telemetering simulator was made with the core development and design of MSP single-chip microcomputer of TI Corporation(Texas Instruments) and RF wireless transceiver chip CC2250 of TI Corporation. The design of the implantation system was optimized to cater to the exploratory experiment on building atrial fibrillation model of New Zealand Rabbits. The implanter was implanted into the abdominal subcutaneous tissue of the New Zealand rabbits, the collected electrodes were placed in the oxter subcutaneous tissues of the left upper extremity and the right upper extremity, and the two simulating electrodes were sutured at the left auricle and the left atrium. The signals were collected and simulated by the wireless transceiver. The I-lead ECG electrical signals were continuously monitored on the body surface by Powerlab physiological recorder. High frequency(the frequency more than 20 Hz) suprathreshold stimulus(strength 2 mA, pulse width1 ms) was emitted by specialized simulation software of a computer program by the interval(simulating for 2s and pausing for 2s). In case of atrial fibrillation during intervals, the simulation could be stopped by human intervention. In case of sinus rhythm, the simulation could be continued. Results The implantable telemetering simulator can work stably in vivo(including collecting simulated electrocardio signal and emitting simulations) for 30 days. Atrial fibrillation can be induced after simulating in vivo of the New Zealand rabbits for 3 weeks, with the duration >48 h. Conclusion Applying implantable telemetering simulator can build the animal model of arrhythmia which is more consistent with welfare optimization and substitution principle for laboratory animals.