The Study On Anti-tumor Effectsof Antisense Oligon Ucleotide In Hela Cells

Backgroud:VEGF-C is a specific lymphatic endothelial cell growth factor,which is expressed in the peri-cancerous tissue,and its role of promoting tumor cell growth and angiogenesis have been reported in more and more literatures.In this study,VEGF-C ASODN was transfected into Hela cells,and its proliferation and migration abilities were examind.Objective: To study the proliferation and migration of antisense oligonucleotide in the Hela cells and research its mechanism.Methods: Hela cells were cultured in vitro and were divided into six group: Control group, Antisense oligonucleotide group(ASODN group), Random sequence group(Random group), Taxol group, Antisense oligonucleotide combined Taxol group(ASODN + Taxol group)and Random sequence combined with Taxol group(Random + Taxol group). The proliferation inhibition of Antisense oligonucleotide in Hela cells was measured by MTT assays. The migration of Antisense oligonucleotide in Hela cells was measured by scratch tests. Expression of VEGFmRNA was measured by PCR. VEGF-C was measured by ELISA.Results: ASDON group could inhibit the proliferation of HeLa cells.The Random group has no significant difference compared with the control group. The proliferation inhibition of ASDON+Taxol group in HeLa cells was higher than that of ASDON group.The results of scratch test showed that antisense oligonucleotide can inhibit the migration of HeLa cells. The migration inhibition of ASODN+Taxol group in HeLa cells was higher than that of ASODN group. Compared with the control group, the ratio of expression of antisense oligonucleotide of mRNA group decreased significantly. ELISA showed that ASODN group led to significant reduction in the levels of VEGF-CConclusion: Antisense oligonucleotide inhibited the proliferation and migration of Hela cells through inhibiting intracellular expression of VECF-C mRNA and secretion of VECF-C.