The Study Of PLGA Controlled Release Microspheres Drug Neuroprotective Effects In Traumatic Brain Injury Rats

Objective To explore the neuroprotective effect of SB-3CT-PLGA release microspheres drug from the apoptosis after traumatic brain injury rats. Method 45 adult SD rats were randomly divided into SB-3CT-PLGA group, SB-3CT group, DMSO control group.Each group was divided to different subgroups(6 hours,48 hours, 7 days after injury), each subgroups had five rats. Using hydraulic shock traumatic brain injury method to make rat models in each group, and then every rat was given tail intravenous injection.The SB-3CT group was according to body weight of rats given 20mg/kg(using DMSO as solvent);The DMSO group was given equal volume of DMSO injection;The SB-3CT-PLGA group was according to the SB-3CT group’s given equivalent drug(with saline prepared into suspension).At different time points after injury rats were killed, the positive cells were detected with TUNEL method. Results All specimens had a lot of positive cells(apoptotic). The SB-3CT-PLGA group,on each time point,the apoptosis index was less than group SB-3CT, and the apoptosis index in SB-3CT group was significantly less than the DMSO group. Conclusions(1)SB-3CT can inhibit the apoptosis of nerve cells in rats after TBI,and have a significant neuroprotective effect on TBI rat;(2)In this experiment,the SB-3CT-PLGA group’s neuroprotective effect is better than the SB-3CT group, we infer that it is may be caused by the sustained release effect on the SB-3CT-PLGA microspheres,which made the local blood drug concentration keep a higher level and prolonged the acting time of effective drug.