HSP70 Family Genes And TNF Alpha Gene Interaction Effect On The Pathogenesis Of The Metabolic Syndrome

Objective To investigate the HSP70 family of genes and TNF-α gene polymorphisms, haplotypes and their interaction with the Ningxia Hui and Han ethnic relationship between the incidence of metabolic syndrome to clarify HSP70 and TNF-а MS susceptibility genes and their interactions on and turn impact of return for finding biomarkers susceptible people with metabolic syndrome prevention, prevention strategies crowd and gene-gene linkage or interaction study theoretical foundation.Method Using a set of case- control study, selected between January 2012 December 2013 at the General Hospital of Ningxia Medical University Affiliated and Wuzhong City People’s Hospital for regular health checks and Ningxia in endocrinology hospital authorities and institutions of the 20- 60-year-old worker in accordance with inclusion and exclusion criteria, to determine the 622 unrelated patients with MS, as the case group, 153 cases in which Muslim men( 24.6%), 156 cases of Muslim women( 25.1%), 161 cases of Han males( 25.9%), 152 cases of Han women( 24.4%), the average age(50.38 ± 6.78) years old, Select from the health check for the same period the population of 600 unrelated healthy people in the control group, Hui and Han ethnic and female, mean age(50.35 ± 6.83) years old,to study subjects in general questionnaire, physical examination and laboratory biochemical parameters, and using Taqman probe assay HSP70-1 gene locus-110, + 190 points, HSP70-hom gene polymorphism loci +2437 and TNF-α gene locus-308; using SNa Pshot method HSP70-2 gene loci detected +2074; HSP70-2 gene +1267 loci detected by PCR direct sequencing.Results HSP70-1 gene-110, + 190 points, HSP70-2 gene locus +1267 + 2074 points, HSP70-hom gene locus +2437, TNF-α gene-308 locus genotype and allele frequencies located in Hui and Han, the differences were statistically significant( all P> 0.05); TNF-α gene locus-308, HSP70-1 gene locus-110, HSP70-2 gene locus +2074, HSP70-hom gene locus +2437 differences in genotype and allele frequencies in case and control groups in each points were significant(all P<0.05), HSP70-1 gene locus +190 genotype and allele frequencies case group and the control group showed no significant distribution( all P> 0.05), HSP70- 2 gene + 1267 sites in each genotype distribution in the case group and control group the difference is significant(P>0.05), allele distribution in the case group and control group have significant difference(P<0.05); HSP70 gene family of +2437,-110, +190, +1267 between the four sites, D’- values between +2074 and +2437 loci were greater than 0.8, that there is a strong linkage disequilibrium between them; The haplotype from + 2074-+2437 loci, haplotype from +2074-+ 2437-- 308 loci, haplotype from-110-+ 190- +1267- +2437 loci, haplotype from-308--110,-+190-+1267-+ 2437 loci were correlated with MS. 4-factor model that composition by +190,+1267,+2437,-308 locis has the highest relative risk of MS.Conclusion HSP70 family of genes-110,+190,+1267,+2074,+2437 points and the TNF-α gene-308 locus genotype and allele distribution in basically the same between Hui and Han nationality; HSP70 family of genes-110,+ 1267,+ 2074,+2437 points and the TNF-α gene polymorphism-308 incidence and Ningxia metabolic syndrome is associated with a significant,-110, +2437 points of C allele, + 1267, +2074 G allele point position and-308 a allele locus mutation increases the risk of MS. Between +2437 and +2074 points, among +2437,-110,+190,+1267 sites between any two of the four sites are in linkage disequilibrium phenomenon. Haplotype C-G, A-T-C, A-T-G, G-C-G, C-A-G-A, A-T-C-C-G, G-C-A-G-A may increase the risk of MS,haplotype T-C, G-T-C, T-A-G-A, G-T-A-G-A may reduce the risk of MS. 4 sites consist of-308,+1267,+2437,+190 locis that interact with metabolic syndrome; +190 locus point unit may no association with MS, but mayby +308- + 1267- 2437 Combination increased susceptibility to MS bit interaction point; +190,+1267 points respectively-308,-110, + 2074 + 2437 can increase the risk of MS interaction point.-110, 2437 points with a synergistic interaction in the pathogenesis of MS. + 2074 and- 308 loci in the incidence of MS interaction with antagonism.