| Purpose: Findings have discovered that the risk of diabetic patients getting macroangiopathy was about 3 times as high as the normal, and that atherosclerosis(AS) accounted for 61.2%. Hui-medicine Compound Artery-malacia Capsule is a Hui-medicine prescription curing DM with atherosclerosis in clinic and with obvious curative effect. Based upon the analysis of four items of lipids(TC, TG, HDL-C and HDL-C), hemorheology level, TEM ultrastructure, the expression of i Nos/PI3K/Akt, the subject further studied the interventions of Hui-medicine Compound Artery-malacia Capsule treating DM complicated with atherosclerosis, which provides the basis for the promotion of Hui-medicine Compound Artery-malacia Capsule in clinic.Methods: 1. Preparation of diabetic rats models: 40 SD rats, all SPF, male, 8-week-old, are randomly divided into normal controls, model controls, male controls, Hui-medicine low-dose group, Hui-medicine high-dose group, each with 8 rats. Except that normal controls feed on general fodder, other rats feed on high-lipid high-sugar fodder to trigger insulin resistance. After a week, rats for modeling fast but drink water, on an empty stomach overnight for 18 h. With intraperitoneal injection as per 45mg/kg according to their body weight, STZ rats continue to be fed on high-sugar high-lipid fodder during this period. Rats in normal controls are intraperitoneally injected with the buffer of sodium citrate under the same conditions. They continue to be fed on general fodder for 8 weeks. 3 days after injection, blood glucose is measured. If random blood glucose(RBG) is measured ≥16.7mmol /L for three times in succession, or such DM symptoms as polydipsia, polyuria, emaciation and weakness develop and RBG is measured ≥11.1mmol /L for two successive times, it is deemed as the success of DM modeling. Rats in successful DM models continue to be fed on high- lipid high-sugar fodder, with free feeding and drinking for 8 weeks. 3 rats are selected according to random number table. They are executed and their thoracic aortas are taken. HE staining hints that atherosclerosis develops, which is deemed as the success of modeling.Results: levels of four items of lipids: serum TC level in normal controls is obviously lower than that in model controls. When Hui-medicine groups are compared with model groups, TC level obviously drops(p<0.01). When rats in model groups are compared with those in normal controls, serum TC level evidently increases(p<0.01). Serum TC levels of Hui-medicine high-dose group(p<0.01) and male controls(p<0.05) are lower than that of model controls. HDL-C level of normal controls is evidently higher than that of model controls(p<0.01). HDL-C level of Hui-medicine high-dose group is evidently higher than that of model controls(p<0.01). LDL-C level of normal controls is evidently lower than that of other groups. LDL-C levels of male controls and Hui-medicine groups obviously increase when compared with model controls(p<0.01).Hemorheology level: when compared with normal controls, low shear rate, middle shear rate and high shear rate of blood viscosity and plasma viscosity of model controls obviously increase(p<0.01). After treatment, blood viscosity values of every group drop evidently when the groups are compared with model controls(p<0.01), where the effect of Hui-medicine high-dose group is obvious(p<0.01).The expression of i Nos/PI3K/Akt: when compared with model controls, the treatment groups can down-regulate i Nos genetic transcription and protein expression, inhibiting PI3K/Akt signaling pathways.TEM observing ultrastructure in different groups: compared with model groups, Hui-medicine groups and male controls can improve ultrastructure of aortic vessel, and the effect of Hui-medicine high-dose group is obvious.Conclusions:1. Hui-medicine Compound Artery-malacia Capsule can control food consumption and increase of weight, and reduce lipids and blood viscosity levels.2. Hui-medicine Compound Artery-malacia Capsule can improve cell ultrastructure and down-regulate i Nos/PI3K/Akt pathways to delay the progress of atherosclerosis.3. Hui-medicine high-dose group is superior to Hui-medicine low-dose group and male controls in terms of the application of lowering lipids and blood viscosity and down-regulating i Nos/PI3K/Akt pathways, with a better effect. |
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