The Study On ID1 In The Carcinogenesis And Progressing Of Salivary Adenoid Cystic Carcinoma

Adenoid cystic carcinoma(ACC) is one of the common malignant tumors in oral and maxillofacial region. Salivary adenoid cystic carcinoma has highly invasion and can recurrence easily after surgery. It’s described as the most destructive and unpredictability tumors in head and neck. Therefore new therapeutic targets finding are crucial for better therapy and prognosis.The full name of ID1 is inhibitor of DNA binding 1. The protein encoded by this gene is a helix-loop-helix(HLH) protein. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may plays a key role in cell growth, senescence, and differentiation. In the study, we discovered the differential expression of ID1 in adenoid cystic carcinoma cell by genetic sequencing technology, and revealed its role and function in carcinogenesis and progressing of salivary adenoid cystic carcinoma. This research can be divided into two parts which are as followed. 1. The differentially expressed gene in salivary adenoid cystic carcinoma and itscorrelations with clinicopathologic characteristics.Aim: To screen the differentially expressed gene in salivary adenoid cystic carcinoma and analyze its correlations with clinicopathologic characteristics.Methods: Extracting the total RNA of SACC-M and SACC-2 and conducting RNA Sequencing by the second generation of genetic sequencing technology in the Encode Genomics Company. We analyzed the RNA sequencing results and screen the differentially expressed gene in SACC cell. To validate the RNA sequencing results, we tested the expression of ID1 in SACC cell by Real-time PCR. To obtain aberrant expression of ID1 in SACC tissue, we employed immunohistochemical staining to study the differences of ID1 expression in 68 cases of salivary adenoid cystic carcinoma tissues and matched 50 cases of normal tissues.Results: Cluster analysis of RNA sequencing results was the upregulated genes including ID1 was concerned with cell locomotion and adhesion in SACC-M when compared with SACC-2. The results of Real-time PCR revealed that ID1 is overexpressed in SACC-M when compared with SACC-2. Immunohistochemical staining results suggest that ID1 is upregulated in salivary adenoid cystic carcinoma tissues when compared with normal tissues.Conclusion: ID1 is specifically overexpressed in SACC tissues, and this upregulation is concerned with metastasis of SACC. 2. The effects of ID1 on the SACC cellAim: To investigate the effect of ID1 on the proliferation, invasion and metastasis of SACC cell.Methods: Using Real-time PCR to measure the expression of ID1 in SACC-M after transfected with si RNA. The proliferation of SACC-M was determined by CCK-8 assay and colony formation assay. The metastasis and invasion were evaluated by transwell migration assay and matrigel invasion assay respectively.Results: Real-time PCR revealed that m RNA level of ID1 was decreased. The results of CCK-8 assay showed the proliferation of SACC-M transfected with si RNA was obviously inhibited when compared with negative control.Colony formation assay suggest that si RNA reduced expression of ID1 and inhibited the growth of SACC-M. Transwell migration assay and matrigel invasion assay revealed that metastasis and invasion of SACC-M were suppressed with the decreasing expression of ID1.Conclusion: ID1 promotes the proliferation, invasion and metastasis of SACC-M.