Detection Of Th17/Treg Cells Balance In Peripheral Blood Of Children With Henoch-schonlein Purpura Combined With Mycoplasma Pneumonia Infection

Objectives:To detect the amount of Th17 cells and Treg cells and the density levels of IL-17, IL-6, TGF-β in peripheral blood of the children in the course of the acute phase of Henoch-Schonlein purpura (HSP) combined with Mycoplasma pneumonia (MP) infection.The children with pure HSP,the children with pure MP infection as well as the normal control group of children were determined.To discuss the correlation between the immune instability of Th17/Treg and the Pathogenesis of the HSP with MP infection.Methods:children in the course of the acute phase of HSP combined with MP-IgM positive (HSP-MP group,18cases,10 males and 8 females), children in the course of the acute phase of HSP combined with MP-IgM negative (HSP group,40 cases,19 males and 21 females), children with MP-IgM positive and non-HSP (MP group,23cases,11 males and 12 females) and 19 healthy children (healthy control group,19cases,10 males and 9 females) were collected. All of the research subjects were collected from August 2012 to June 2013 in our hospital. Four groups were measured the quantity of Th17 cells and Treg cells of peripheral blood by flow cytometry,using ELISA(enzyme-linked immunosorbent assay, ELISA)assay the density levels of IL-17, IL-6, TGF-β in peripheral blood of the four groups.Result:1.The percentage of Th17 cells and the density levels of IL-17 and IL-6 in HSP-MP group were significantly higher than the other three groups (P<0.05).The quantity of Th17 cells and the density levels of IL-17 and IL-6 in HSP group and MP group were both significantly higher than the normal control groupP <0.05).There was no significant difference of the quantity of Th17 cells and the density levels of IL-17 and IL-6 between HSP group and MP group(P>0.05).2.The quantity of Treg cells in HSP-MP group were significantly lower than the other three groups(P<0.05).The quantity of Treg cells in HSP group and MP group were both significantly higher than the normal control group(P<0.05).There was no significant difference of the quantity of Treg cells between HSP group and MP group(P>0.05).3.There was no significant difference of the the levels of TGF-β among the four groups.(P>0.05).Conclusion:1.More severe Th17/Treg cells immune disorder may play an important role in the pathogenesis of HSP combined with MP infection of children.2.The more severe Th17/Treg cells immune disorder may be caused by the higher level of IL-6 in peripheral blood.