| Objective By studying the effects of Helicobacter pylori on the expression of helper T cells 17(Th17),CD4+ CD25+ Foxp3+ regulatory T cells and related cytokines interleukin-10,IL-22,IL-10 expression levels in peripheral blood of children with Henoch-Schonlein purpura and exploring the influence of H.pylori infection on the immune response of children in the acute phase of HSP,and further explore the mechanism of the occurrence and development of HSP,so as to provide new ideas and new methods for further immunotherapy.Methods The patients selected from the Department of Pediatrics of our hospital from January 2016 to June 2017 to be hospitalized for the first diagnosis of HSP in children,a total of 70 cases as experimental group,including 36 males and 34 females,aged from 3 years to 10 years.And they all conform to the diagnostic criteria of HSP produced by the 2006 European League Against Rheumatism and Pediatric Rheumatology Society classification criteria.In the normal control group,there were 30 healthy children in the same period.There were 14 males and 16 females,aged from 3 years to 11 years old.The two groups of children were comparable in age and sex,and the difference was not statistically significant.The rapid urease,C13 breath test and slice staining were used to detect the presence of H.pylori infection in both groups,and HSP children were divided into H.pylori positive subgroup and H.pylori negative subgroup.The proportion of Th17 and Treg in CD4 + T cells in peripheral blood was detected by flow cytometry.The levels of IL-17,IL-22 and IL-10 in serum of each group were detected by enzyme linked immunosorbent assay(ELISA).Results(1)Compared with the normal control group,the HSP group had 36 males and 34 females,with an average age of(8.5±3.1)years and in the control group,16 males and 14 females,with an average age of(7.9±2.3)years.There was no significant difference in gender and age between the two groups of children(P>0.05).(2)The positive rate of H.pylori in HSP group was 65.7%(46/70),which was significantly higher than that in control group(16.7%,5/30)(P<0.05).(3)The percentage of Th17 in CD4 + T cells in HSP group was(2.72± 0.46)%,which was significantly higher than that in normal control group(1.33± 0.40)%,the difference was statistically significant(P<0.05).The proportion of Th17 in CD4 + T cells in H.pylori positive subgroup was(3.05±0.51)%higher than that in H.pylori negative subgroup(1.98±0.25)%,and the difference was statistically significant(P<0.05).(4)The percentage of Treg cells in CD4 + T cells in HSP group was(2.31 ±0.42)%,which was significantly lower than that in normal control group(6.79±2.23)%,and the difference was statistically significant(P<0.05).The percentage of Treg cells in CD4 + T cells in H.pylori positive subgroup was(1.10±0.21)%,lower than that in H.pylori negative subgroup(3.07±1.15)%,and the difference was statistically significant(P<0.05).(5)The levels of IL-17 and IL-22 in the HSP group were(32.79±6.98)and(20.52±2.74),respectively,which were significantly higher than those in the control group(18.76±4.21),(8.52±2.26),the difference was statistically significant(P<0.05).The level of IL-10 in HSP group was(55.32±6.38),which was significantly lower than that of the normal control group(78.20±6.54),and the difference was statistically significant(P<0.05).The difference was statistically significant(P<0.05).The levels of IL-17 and IL-22 in H.pylori positive subgroup were(40.85±6.58)and(25.24±3.46),respectively,which were significantly higher than those in H.pylori negative subgroup(30.12±3.18)and(18.24±5.26)).The difference was statistically significant(P<0.05).The positive rate of IL-10 in H.pylori positive subgroup was(48.72±5.37),lower than that in H.pylori negative subgroup(59.44±4.59),the difference was statistically significant(P<0.05).The positive rate of IL-10 in H.pylori positive subgroup was(48.72±5.37),which was lower than that in H.pylori negative subgroup(59.44± 4.59),the difference was statistically significant(P<0.05).Conclusions(1)There is a potential association between H.pylori and HSP,and the positive rate of H.pylori in group HSP is higher than that of normal children.(2)Flow cytometry and enzyme-linked immunosorbent assay showed that Th17 and related cytokines IL-17 and IL-22 were highly expressed in the peripheral blood of HSP,while Treg cells and related cytokines IL-10 were low expressed in the peripheral blood of HSP.(3)H.pylori can aggravate the immune dysfunction of HSP.Compared with H.pylori negative HSP patients,the expression of Th17 and related cytokines increased and the expression of Treg cells and related cytokines decreased in HSP patients with H.pylori infection.(4)It is possible to explore the role of H.pylori in the pathogenesis of HSP and to treat it with targeted treatment,which can achieve the purpose of treating or alleviating the disease. |
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