The Antibacterial Activity Of Acetic Acid Against The Pathogens Of Relevance To Chronic Wounds And The Toxic Effects On Murine Fibroblast Cell Line

Objective:To study the antibacterial activity of acetic acid against the pathogens of relevance to chronic wounds, inquiry experiment under the condition of minimum bacteriostatic concentration of acetic acid with different strain;preliminary exploration of the same volume fraction of the organic acid and inorganic acid bacteriostatic ability;at different concentration of acetic acid in mice fibroblast toxicity time curve;morphology research effective bacteriostasis concentration of acetic acid for methicillin-resistant staphylococcus aureus and verdigris false single spore bacteria bacterial biofilm. Method:After purification, develop chronic wound common bacteria types and their standard strains, using spectrophotometer to detect effect of different concentration of acetic acid and hydrochloric acid, acetic acid and analyze the experimental conditions inhibit bacterial growth, the minimum bacteriostasis concentration;conventional methods to cultivate the mice fibroblast(L929), detected by the MTT method of different concentrations of acetic acid in mice fibroblast toxicity;static culture of methicillin-resistant staphylococcus ball and prepared by the bacteria pseudomonas aeruginosa biofilm, laser confocal microscope effective bacteriostatic concentration of acetic acid for methicillin-resistant staphylococcus ball and bacteria pseudomonas aeruginosa biofilm formation and mature the influence of bacterial biofilm. Results:1. The acetic acid inhibition of standard strains of e. coli(ATCC 25922), the minimum bacteriostasis concentration was 0.63%, hydrochloric acid is 0.32%;2. The acetic acid inhibition of clinical drug resistant strains of e. coli, the minimum bacteriostasis concentration was 0.63%, hydrochloric acid is 0.32%;3. The acetate inhibit staphylococcus aureus standard strains(ATCC 25923), the minimum bacteriostasis concentration was 1.25%, hydrochloric acid is 0.32%;4. Acetic acid suppression of methicillin-resistant staphylococcus aureus, the minimum bacteriostasis concentration was 1.25%, hydrochloric acid is 0.32%;5. Acetate inhibit pseudomonas aeruginosa standard strains(ATCC 27853), the minimum bacteriostasis concentration was 0.63%, hydrochloric acid is 0.32%;6. Acetic acid inhibition of clinical drug resistant strains of pseudomonas aeruginosa minimum bacteriostasis concentration was 0.32%, 0.32% hydrochloric acid.7. Acetic acid inhibition of clinical drug resistant strains of acinetobacter baumannii minimum bacteriostatic concentration is 0.32%, and 0.32% hydrochloric acid.8. Acetic acid in mice fibroblast cell toxicity test showed that the concentration of 0.32% and 0.63% acetic acid effect after 4 h- 48 h, not significant effects on the cell growth;The concentration of 1.25% acetic acid effect time is more than 12 h, in mice fibroblast growth had a significant effect.9. Laser confocal microscopy observation results show that the volume fraction of 0.63% acetic acid can effectively destroy staphylococcus aureus and verdigris false single spore fungus clinical strains of bacterial biofilm matrix structure, mature, in turn, to inhibit the growth of bacteria, and the volume fraction of acetic acid in staphylococcus aureus and verdigris false single spore fungus clinical strains of bacterial biofilm formation damage in the process of biofilm matrix structure, reduce the number of living bacterium, to inhibit the growth of bacteria. Conclusion:0.32-1.25% concentration of acetic acid can inhibit the growth of common bacteria on burn wounds.And cell toxicity test showed that the concentration of 0.32 to 0.63% acetic acid effect after 48 hours, not the growth of fibroblasts in mice have a significant impact, the concentration of 1.25% acetic acid effect time is more than 12 hours later, began to inhibit the growth of fibroblasts in mice, and with the duration extension, inhibition enhanced obviously;Compared with concentration of hydrochloric acid and acetic acid, for the inhibition of bacteria is higher than acetic acid;The volume fraction of 0.63% acetic acid can effectively destroy staphylococcus aureus and verdigris false single spore fungus clinical strains of bacterial biofilm matrix structure, mature, in turn, to inhibit the growth of bacteria, and the volume fraction of acetic acid in staphylococcus aureus and verdigris false single spore fungus clinical strains of bacterial biofilm formation damage in the process of biofilm matrix structure, reduce the number of living bacterium, to inhibit the growth of bacteria.