Ginsenoside Rg1 Has Impacts On Exhaustive Exercise Rats Of Skeletal Muscle And Brain Tissue Carbonylation Protein Content

1 Objective The experiment combining the theory of free radical fatigue and carbonyl stress research, by using the exhausted fatigue model. In order to understanding moderate-intensity exercise for 4 weeks or 8 weeks and combination Ginsenoside Rg1 of gavage impact on the exhausted state of skeletal muscle and brain tissue protein carbonyl content in rats. From the perspective of suppressing carbonyl stress, the preliminary discussion the Ginsenoside Rg1 for mechanism of suppressed exercise-induced fatigue.2 Materials and methodsSubjects for 40 SD rats of clean grade, were randomly divided into quiet group for 4 weeks(Q4), exercise group for4 weeks(E4), quiet group of gavage Ginsenoside Rg1 for 4 weeks(QR4), exercise group of gavage Ginsenoside Rg1 for 4 weeks(ER4), quiet group for 8 weeks(Q8), exercise group for 8 weeks(E8), quiet group of gavage Ginsenoside Rg1 for 8 weeks(QR8),exercise group of gavage Ginsenoside Rg1 for 8 weeks(ER8),each group 5 only. Rats for ER group and QR group are given by gavage Ginsenoside Rg1 solution with 30 mg/kg body weight dose. Rats for exercise group and quiet group are given by gavage normal saline of identical volume. Rats for E group and ER group are forced to accept cycle treadmill exercise for 4weeks or 8 weeks with the load of 25 m·min-1×30min. After the scheme, it is time to recording exhausts time and drawing materials immediately. Determination of blood lactic acid(LA)content in serum of rats; Determination of skeletal muscle brain tissue for the super oxide dismutase(SOD) activity and catalase(CAT) activity and glutathione peroxidase(GSH-Px)and malondialdehyde(MDA) content and carbonylation protein content.3 Research results3.1 Weight determination resultsAfter the experiment, SD rats of body weight in each group were significantly increased; E4 Group and ER4 group were significantly lower than QR4 group(P <0.05); E8 group and ER8 group were significantly lower than Q8 group(P <0.05), QR8group(P <0.01); the rest had no significant difference.3.2 Exhausts Time determination resultsCompared with Q4, E4 group and ER4 group were significantly prolonged(P <0.01); Compared with QR4 group, ER4 group was significantly prolonged(P <0.01); Compared with the E4 group,ER4 group was significantly prolonged(P <0.01). Compared with Q8 group, E8 group and ER8 group were significantly prolonged(P <0.01); Compared with QR8 group, ER8 group was significantly prolonged(P <0.01). Compared with E8 group, ER8 group was significantly prolonged(P <0.01).E8 group was significantly longer than the exercise E4group(P <0.01), ER8 group was significantly longer than ER4group(P <0.01). The rest had no significant difference.3.3 Blood lactate determination resultsCompared with Q4 significantly lower E4 Group and ER4 group(P <0.01); and QR4 group, significantly lower ER4 group. And Q8 group, significantly lower E8 group and ER8 group(P <0.01);and QR8 group, ER8 group was significantly lower(P <0.01).The rest had no significant difference.3.4 Antioxidant enzymes activity and MDA and carbonylation protein content in rat skeletal muscleCompared with Q4, E4 group CAT activity was significantly increased(P <0.05), MDA and carbonyl protein content significantly decreased(P <0.01); Compared with QR4 group,ER4 group SOD activity, GSH-Px activity and CAT activity were significantly increased(P <0.05), MDA and carbonyl protein content were significantly decreased(P <0.05); Compared with E4 group, ER4 group SOD activity was significantly increased(P <0.05), MDA content was significantly decreased(P <0.05);Compared with Q8 groups, E8 group GSH-Px and CAT activity were significantly increased(P <0.01, P <0.05), MDA and carbonyl protein content were significantly decreased(P <0.01);Compared with QR8 group, ER8 group SOD 、GSH-Px and CAT activity were significantly increased(P <0.01), MDA and carbonyl protein content were significantly decreased(P <0.01);Compared with the E8 group, ER8 group SOD activity(P <0.01),GSH-Px activity and CAT activity were significantly increased(P <0.05), MDA and carbonyl protein content were significantly decreased(P <0.05). ER8 group SOD activity were significantly higher than ER4 group(P <0.05); E8 group GSH-Px activity was significantly higher than E4 group(P <0.05), ER8 group was significantly higher than ER4 group(P <0.05). The rest had no significant difference.3.5 Antioxidant enzymes activity and MDA and carbonylation protein content in rat brain tissueCompared with Q4, E4 group carbonyl protein content was significantly decreased(P <0.01). Compared with QR4 group,ER4 group SOD and CAT activity were significantly increased (P <0.01, P <0.05), MDA and carbonyl protein content was significantly decreased(P <0.05, P <0.01); Compared with the E4 group, ER4 group SOD activity was significantly increased(P <0.01). Compared with Q8 group, E8 group SOD activity(P<0.01), GSH-Px activity(P <0.01) and CAT activity(P <0.05)were significantly increased, MDA and carbonyl protein content were significantly decreased(P <0.01). Compared with QR8 group, ER8 group SOD,GSH-Px and CAT activity were significantly increased(P <0.01), MDA and carbonyl protein content were significantly decreased(P <0.01). Compared with E8 group, ER8 group SOD and GSH-Px activity were significantly increased(P<0.01, P<0.05), MDA and carbonyl protein content were significantly decreased(P <0.05).ER8 group SOD, GSH-Px and CAT activity were significantly higher than ER4 group(P <0.05); ER8 group MDA content was significantly lower than ER4 group(P <0.05). The rest had no significant difference.4 Conclusion4.1 It could be improved Exercise capacity and prolonged exhausts time by Long-term exercise training or Combining with ginsenoside Rg1;4.2 Long-term exercise training can enhance the activity of antioxidant enzymes and improve ability of against oxidative stress and carbonyl stress,reducing carbonylation protein generated;4.3 Ginsenoside Rg1 can enhance activity of antioxidant enzymes in motion rats and improve ability of against oxidative stress and carbonyl stress,reducing carbonylation protein generated.