Influences Of TGF-β₃ On Proliferation And Osteogenic Differentiation Of Rabbit Dental Pulp Stem Cells

Objective:To investigate the influence of TGF-β3 on the proliferation and differentiation of rabbit dental pulp stem cells. Methods: DPSCs were isolated with tissue enzymedigestion method. The third passage DPSCs were chosen and divided into five groups, ncubated with 0 ng/m L, 20 ng/mL, 40 ng/mL, 80 ng/m L, and 100 ng/m L TGF-β3 respectively. MTT assay were performed to investigate the A490 values respectively. Immunocytochemistry staining of OC, Col-I, BSP and Alizarin red staining were performed respectively. Results: DPSCs successfully formed cell unit in vitro, A490 values were 0.34±0.55, 0.34 ± 0.19, 0.33 ± 0.47, 0.33 ± 0.30, 0.34±0.47 respectively, there were no significant difference(P>0.05). In group 80 ng/m L and 100ng/m L TGF-β3, Col-I were positive on the day 3, and began to disappear on the day7. Bsp were positive on the Day 5 to 7, OC were positive on the day 7 to 14, and Alizarin red staining were positive on the day 7. In group 40 ng/m L TGF-β3, Col-I was positive on the day 5 to 7, BSP was positive on the day 7, OC and Alizarinred staining was positive on the day 14. In group 20 ng/m L TGF-β3, Col-I was positive on the day 7, and began todisappear on the day 7. BSP was positi-ve on the day 14, OC and alizarin red staining was positive on the day 14. We detected that there were no positive results in the control group. Conclusion: TGF-β3 has no influence on the proliferation, of DPSCs, while accelerate the osteogenic differentiation of DPSCs in adose dependent manner.