The Influence Of MiR-21 Control Smad7 Expression And Lung Cancer A549 Cell Line Proliferation

ObjectiveThe incidence of lung cancer is a complex process involving environmental, genetic effects and the accumulation of genetic events and host tumor interactions, eventually leading to uncontrolled cell growth and cloning of tumor development.Our aim is to explore miR-21 regulation of Smad7 in lung cancer A549 cell line as well as its impact on the proliferation of lung cancer A549 cell line.To analyze the mechanism of miR-21 and Smad7 in the development of non small cell lung cancer, and to understand the pathogenesis of lung cancer, and to provide evidence for the treatment of lung cancer.MethodsA549 cells were cultured in the conventional culture of lung cancer cell line A549, and the transfection experiment was carried out in the logarithmic growth phase. The miR-21 inhibitor, simulation and negative control and lipid body Lipofectamine TM 2000 reagent mixing, standing at room temperature for 20 min after the addition of the corresponding hole cells in and 37 DEG C,5% CO2 insulation incubator breeding 4-6 hours, replacement of serum containing RPMI1640,24 h and 48 h after extraction after transfection cell total RNA and total protein. MTT group was transfected with miR-21 inhibitor group, transfected with miR-21 analog and negative control group, the cell proliferation was detected by colorimetric assay. Blot RNA and qRT-PCR were used to detect the expression level of Smad7 gene and protein after Western extraction. The proliferation of A549 cells was detected by MTT method.ResultsSimulations with transfection of miR-21 after Smad7 mRNA and protein expression was decreased (P<0.05), and after the transfection of miR-21 inhibitor Smad7 mRNA and protein expression increased (P<0.05); MTT assay showed that transfection of miR-21 simulation after the proliferative capacity of the cells increased significantly (P<0.05), and after transfection of miR-21 inhibitor of cell proliferation significantly weakened (P<0.05).ConclusionmiR-21 inhibitors can increase Smad7 protein and mRNA expression, and suppress the proliferation activity of lung cancer A549 cell significantly.