| The purpose of this paper is to study the protective actions of royal jelly in liver injury of mice, which is caused by Carbon tetrachloride (CCl4), and to further discuss its protective mechanisms. The main contents and results of this research are as follows:1. Liver injury induced by CCI4 in mice was established. We discovered the protective effects of royal jelly on hepatic injury in mice by CCl4 from the liver index, thymus index, spleen index and clinical indexes, such as transaminase, triglyceride, total bilirubin and alkaline phosphatase and liver tissue pathological. The results showed that alanine aminotransferase and aspartate aminotransferase levels of model group significantly increased (P<0.01) compared with normal group, which meant it was a successful animal model. The liver index of model group was also significantly higher than that in other groups (P<0.01), which also suggested that the model was successful. But the liver index of royal jelly groups were remarkable lower than model group (P<0.01), the thymus and spleen index were significantly higher than model group (P<0.01). The content of triglyceride, total bilirubin and alkaline phosphatase of royal jelly groups were significantly lower than model group (P<0.05), indicating that royal jelly had a good protective effects on liver injury induced by CCI4. The results of liver tissue pathological also further confirmed the above results.2. By measuring the activity of anti-oxidant enzymes which includes superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), peroxidase (CAT) and total antioxidant capacity (T-AOC) and the level of lipid peroxidation and some anti-inflammatory factors the of liver-injury mice induced by CCl4, we studied anti-oxidant mechanism of royal jelly on liver injury caused by CCl4. The results indicated that the content of malondialdehyde of model group was significantly increased (P<0.01) compared with normal group, but the activity of every anti-oxidant enzyme was significantly decreased (P<0.01). Compared with model group, the activities of anti-oxidant enzymes were significantly higher (P<0.01), and the contents of malondialdehyde, IL-1β and TNF-α were significantly lower (P<0.01).3. We studied motigen-activated protein kinase signal pathways by western blot, in the process of which royal jelly protected the liver injury induced by CCl4. We measured expression quantity of three kinds of protein in the signal path way, which contained JNK/SAPK, p38MAPK and p42/44 MAPK by using western blot. The results showed that the contents of these proteins in model group were significantly higher than those in other groups (P<0.05). And the content of these proteins in other groups were significantly lower than that in model group (P<0.01). The content of these proteins in royal jelly groups were significantly lower than that in positive group (P<0.01). These results proveded that this signal pathway involved in liver injury induced by CCl4, and royal jelly could inhibit its expression.We can conclude from above results as follows:royal jelly could effectively improve the physiological indicators of liver injury in mice, enhance the body’s immune function in mice, and have a certain role in promoting the repair of liver tissue structure, and could enhance the body’s antioxidant ability, inhibite lipid peroxide process, decrease the mice serum level of IL-1β and TNF-α, inhibit the expression of motigen-activated protein kinase signal pathway, so as to achieve the purpose of protecting the liver. |
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