The Effect Of AML1-ETO On DNA Repair Function And Preliminary Study On Its Mechanism

【Objectives】To study the effect and mechanisms of AML1-ETO on DNA repair function in vitro.【Methods】After transfected the plasmid with AML-ETO、PML-RARa,K562、HL60、293T cells are treated with 4Gy irradiation.Continued cultured 30 minutes,Western blot and Real-time PCR are used to test the DNA repair related protein.【Results】Compared with the control group in AML1-ETO overexpression cells,γH2AX(a DNA double strand break marker) is similar.One of the repairing related protein XLF is increased in AML1-ETO overexpressed cells after treated with 4 Gy irradition,whether in K562、293T、HL60 cells.Although DNA-PK、KU80(non-homologous end joining(NHEJ) repair related protein) are increased than the control group,but both the AML1-ETO and PML-RARa overexpressed cells are all increased.Similar result is shown in P-ATM(one of homologous recombination(HR) repair related proteins).No change are shown for other HR repair related proteins,such as MRE11、RAD50、NBS1 in experiment teams and control.【Conclusions】1.AML1-ETO can increase the ability of DNA repair, the main effect of NHEJ repair methods.2. AML1-ETO main influence NHEJ related proteins in XLF.