Study On Chemical Constituents Of Artemisia Capillaris

Artemisia capillaris, which belongs to the Asteraceae perennial herbs,called wormwood, Astemisia Scopatia, continous wormwood, Velvet Artemisia, Fine leaf artemisia annua, wild orchid artemisia, etc. It has been recorded in Shen Nong’s herbal classic and Compendium of materia medica. Artemisia capillaries has a variety of use in medicinal and edible and relatively rich resources, is widely distributed in our country. People picking leaf of artemisia capillaris medicine Artemisia capillaris has extensive pharmacological effects. Such as protect liver, bravery, antitumor, antibacterial, fall blood pressure, antipyretic and analgesic, anti-inflammatory and asthma, etc.This paper summarizes the two aspects of the research status of Artemisia capillaris on pharmacological effects and the chemical composition in domestic and international. DPPH radical scavenging activity and Tyrosinase inhibitory activity are used as a guide. According to activity guide, Artemisia capillaris was isolated from the ethyl alcohol extract Artemisia capillaries. The silica gel column chromatography, ODS reversed phase column chromatography and semi-preparative HPLC and modern chromatographic methods were used to isolate the ethyl alcohol extract of the Artemisia capillaris, and 22 compounds were isolated. And then the structure of the 12 compounds were indentified by nuclear magnetic resonance, mass spectroscopy and other modern analysis methods.Twelve compounds were carried out the experiment of the DPPH radical scavenging activity and Tyrosinase inhibitory activity. The results show that, The compound 7,8,9,14,19 toward DPPH radical scavenging activity have an ability of the most prominent.The IC50 value of DPPH radical scavenging activity of compound 7,8,9,14,19 is 1.54 μg/mL,0.42 μg/mL,2.28 μg/mL,2.90 μg/mL,2.04 μg/mL. The compound 7,8,9,14,19 is less than the IC50 value of vitamin C reference(4.52 μg/mL).