The Study Of Extraction And Seperation Of Key Medicinal Components From Schisandra

Schisandra Chinensis, which contains lignans, schisandrae polysaccharide, edible pigment,and many other medicinal components, is a kind of dry fruit of magnoliaceae plant of fructus schisandrae. Different components have different medicinal function, thus will be to identify the extraction and separation of components is of great significance.This subject adopts the method of direct extraction respectively, examines the leaching rates of lignans, polysaccharide and anthocyanin, soxhlet extraction of lignans, as well as the kinetics, adsorption isotherms and thermodynamics of resins for adsorption of anthocyanins.Besides, the structures of polysaccharide and pigment, seperation technology and the scale-up experiment were also studied.Here, influence of process conditions on the total leaching rate, the leaching rates of lignans, polysaccharide and anthocyanin were studied and the optimum conditions were confirmed by comparing relative leaching rate. The results showed that the optimum conditions of total lignans were the leaching time of 16 h, the leaching temperature of 50℃,and the solid-liquid ratio of 25mL/g. While the optimum conditions of schisandrin were 16 h,30℃ and 25mL/g, respectively.Soxhlet extraction of lignans was studied in this work. The factors of siphonate numbers,ratio of liquid to solid, particle size and ethanol concentration were investigated in detail and the response surface experiment was designed and carried out to optimize the process parameters. The results showed that the optimum conditions for schisandrin extraction were the ethanol concentration of 98.05%, the ratio of liquid to solid of 67.20mL/g, and the average particle size of 0.33 mm. An optimum extraction rate of 80.90% was obtained under the conditions, and the result obtained from experiment was 77.17%. For deoxyschizandrin,the optimum conditions were 98.05%, 67.27mL/g and 0.33 mm, respectively. An optimum extraction rate of 77.11% was obtained, and the result from experiment was 74.31%. The ethanol concentration of 96.38%, the solid-liquid ratio of 72.52mL/g and the average particle size of 0.21 mm were tfor schisandrin B and its optimum extraction rate ran up to 77.13%,and the result from experiment was 74.23%. Meanwhile, the significance of the factors on the extraction rate of the lignans was as following: ethanol concentration > solid-liquid ratio >particle size.The kinetics, adsorption isotherms and thermodynamics of resins for adsorption of anthocyanins were studied. The kinetic data was found to fit the pseudo-second-order kinetic model better. The equilibrium data of anthocyanins was found to follow the Freundlich adsorption isotherm. The values of thermodynamic parameters including the changes of Gibbs free energy(ΔG), entropy(ΔS) and enthalpy(ΔH) demonstrated the process ofadsorption was spontaneous and endothermic. The adsorption process was controlled by physical mechanism rather than chemical mechanism. Besides, the structures of polysaccharide and pigment, seperation technology and the scale-up experiment were also studied in this part to give guidance to the production of up-scale.